Your Manuscript should include:
A) Title Page
B) Abstract Page
C) Introduction
D) Materials and Methods
E) Results and Discussion
F) Literature Cited
G) Acknowledgement
H) Statement of Authors' Contributions to Manuscript
I) Required forms

A) TITLE PAGE: The title page must include:

1. The title should be composed as a single declarative statement. The title should be focused on the results presented in the manuscript. Please do not use a colon or semicolon in the title. Please keep the title as generally applicable as possible. It usually is not necessary to include the exact study location or a specific study name in the title, as this information can be included in the abstract.
2. The names of all authors (first name, middle initial, last name) including their departmental and institutional addresses. Indicate which authors are associated with which institutions by numbered footnotes. Identify a corresponding author and provide a complete mailing address, telephone number, fax number, and email address. Please note that all authors’ names should appear on the manuscript exactly as they should appear in PubMed if the paper is published. ASN will not replace files to correct author names once published.
3. The last name of each author for the purpose of PubMed indexing
4. The word count for the entire manuscript (title through references). See word limit above.
5. The number of figures.
6. The number of tables.
7. Whether supplementary online material has been submitted.
8. A running title of 48 characters or less.
9. Footnotes to the title disclosing: (a) all sources of financial support; (b) all potential conflicts of interest; (c) the existence of online supporting material, if appropriate (see section on Online Supporting Material).

   Conflict of Interest and Funding Disclosure: Any existing financial arrangements between an author and a company whose product figures prominently in the submitted manuscript or between the author and any company or organization sponsoring the research reported in the submitted manuscript should be brought to the attention of the Editor in the cover letter that accompanies the manuscript submission. In addition, all authors must declare all sources of funding for research reported in their manuscript and report all potential conflicts of interest in separate footnotes on the manuscript title page. If an author has no conflicts of interest, the footnote should list the author’s name, followed by “no conflicts of interest”. A conflict of interest includes, but is not limited to:

   • Any existing financial or personal interests with a company whose product figures prominently in the submitted manuscript
   • Any financial or personal interests with any company or organization sponsoring the research reported in the submitted manuscript
   • Financial or personal interests include: a current grant, contract or subcontract, or consulting agreement with a company; employment with the company/organization; acting as an expert witness on behalf of a company/organization; holding stocks or shares in a company
   • Coordinators of supplement publications should also report any financial and personal interests, as defined above, with sponsors of supplement publications. In addition, supplement coordinators should disclose receipt of compensation from sponsor for editorial services on manuscripts published in the supplement publication and/or for attending, speaking or organizing a meeting or symposium
   
   Individuals who are asked to review a manuscript should decline the solicitation if they have:
   (1) served as an adviser or advisee to an author on the manuscript
   (2) collaborated or served as a coauthor with an author of the manuscript during the past 3 years;
   (3) are currently affiliated with, were previously employed within the past 12 months by, or are being considered for employment at the institution of an author;
   (4) participated in a consulting/financial arrangement with an author in the past 3 years; or
   (5) are the spouse, child, sibling, parent, partner, or close friend, or otherwise have a relationship that might affect judgment, or could be seen as doing so by a reasonable person familiar with the relationship.

C) INTRODUCTION: Background to the research conducted and specific objectives should be clearly indicated. This should not be a comprehensive review of the literature, however.

D) MATERIALS AND METHODS: Documentation of methods and materials used should be sufficient to permit replication of the research. State the source of specialized materials, diets, chemicals, and instruments and other equipment, with model or catalog numbers, where appropriate. Specify kits, analyzers, and commercial laboratories used. Cite references for methods whenever possible and briefly explain any modifications made.

HUMAN AND ANIMAL RESEARCH. Reports of human studies must include a statement that the protocol was approved by the appropriate institutional committee or that it complied with the Helsinki Declaration as revised in 1983. When preparing reports of randomized, clinical trials, authors should refer to the checklist published in the CONSORT Statement and should include as online supporting material a trial profile summarizing participant flow (2). Include in Methods the sizes (n) of initial (recruited, enrolled) and final groups. Research on animals should include a statement that the protocol was approved by the appropriate committee or complied with the Guide for the Care and Use of Laboratory Animals (3). Describe how animals were killed. Describe control and experimental subjects giving age, weight, sex, race, and for animals, breed or strain. Include the supplier of experimental animals.

DIETS. Composition of control and experimental diets must be presented. When a diet composition is published for the first time in The Journal of Nutrition, utilize a table or a footnote to provide complete information on all components. If previously described in The Journal of Nutrition or The American Journal of Clinical Nutrition, a literature citation may be used. State specifically any modifications made to the published diet compositions. The proximate composition of closed formula diets should be given as amounts of protein, energy, fat, and fiber. Components should be expressed as g/kg diet. Vitamin and mineral mixture compositions should be included using Journal of Nutrition units and nomenclature. For a discussion of the formulation of purified animal diets, refer to Baker (4) and to a series of ASN publications (5-8).

STATISTICAL METHODS. Describe all statistical tests utilized and indicate the probability level (P) at which differences were considered significant. If data are presented in the text, state what they represent (e.g. means ± SEM). Indicate whether data were transformed before analysis. Specify any statistical computer programs used.

Present the results of the statistical analysis of data in the body of each and on figures per se. Use letters or symbols to indicate significant differences; define these in a table footnote or the figure legend. Provide the appropriate statistics of variability. An estimate of the error variance (SD or SEM) of group means should be displayed in figures. Standard ANOVA methodology assumes a homogeneous variance. If error variance is tested and found to be heterogeneous, data should be transformed before ANOVA, or nonparametric tests should be used. For a discussion of variability calculations and curve-fitting procedures, see Baker (4).

E) RESULTS AND DISCUSSION Report the results of the study. Discuss the significance of the findings, interpret the results and conclusions.

F) LITERATURE CITED The Journal of Nutrition reference format will be modified to be consistent with the International Committee of Medical Journal Editors (ICMJE) recommended format for bibliographic citations with the following exception: references should include the names of all authors, unless there are more than ten, in which case list the first nine plus "et al." There is no limit on the number of citations allowed; recent literature should be comprehensively cited. The list of references must begin on a new page and should include the heading "Literature Cited." Abbreviate journal names according to the National Library of Medicine (NLM) journal abbreviations list . References should be numbered consecutively in the order in which they are first mentioned in the text.

References in tables and figures: References cited for the first time in tables or figure legends should be numbered in order, based on the placement of the table or figure in text. Identify references in text, tables, and legends for illustrations by arabic numbers in parentheses. See current print issues of The Journal of Nutrition for style.

Make sure your Literature Cited section includes a recognized heading and that the heading is not set in all caps (use upper and lower case letters, as shown below). Recognized headings include the following:

References

Reference List

Literature Cited

References and further reading

Bibliography

Literature

G) ACKNOWLEDGMENTS. Technical assistance and advice may be acknowledged in a section at the end of the text. Only named individuals should be included in this section. Authors are responsible for obtaining written permission from everyone acknowledged by name and for providing copies of signed permission statements to The Journal of Nutrition. These statements should be submitted to The Journal along with the manuscript Authors' Statement/Copyright Release Form. Also see below, H) STATEMENT OF AUTHORS' CONTRIBUTIONS TO MANUSCRIPT.

H) STATEMENT OF AUTHORS' CONTRIBUTIONS TO MANUSCRIPT. Effective July 1, 2009, authors must indicate their contribution(s) to the manuscript in the Acknowledgments section. Use the descriptors listed below unless the author performed a function that clearly is not covered by one of these. While not all manuscripts will necessarily include all descriptors, all manuscripts, including reviews, must indicate who is responsible for design, writing, and final content.

1. designed research (project conception, development of overall research plan, and study oversight.)
2. conducted research (hands-on conduct of the experiments and data collection.)
3. provided essential reagents, or • provided essential materials (applies to authors who contributed by providing animals, constructs, databases, etc., necessary for the research)
4. analyzed data, or, • performed statistical analysis
5. wrote paper (only authors who made a major contribution.)
6. had primary responsibility for final content
7. other (use only if categories above are not applicable; describe briefly) .
8. For single-authored research papers and reviews, please state: The sole author had responsibility for all parts of the manuscript.
   
   Please do not include “obtained funding” (the initials of authors who received grants may be included in the footnote regarding Support).
   
   An example is: A. X., R. F. G., and P. G. Y. designed research; R.F. G. and Q. C. conducted research; P. T. analyzed data; A. X., P. G. Y. and Q. C. wrote the paper. P. G. Y. had primary responsibility for final content. All authors read and approved the final manuscript.

UNITS OF MEASURE. Most measurements must conform to le Systeme Internationale d'Unites (SI) (9). The metric system and the Celsius scale (º C) must be used. Concentrations should be expressed on a molar basis. Except for diet composition, convert to substance concentration, e.g., mol/L. The denominator should be L. Do not use M, mM, N, etc. Use one of three acceptable options to express measurements. (a) Use SI units exclusively. (b) Use SI units and, if appropriate, provide conventional units parenthetically in the text and give conversion factors in table footnotes and figure legends. (c) Use conventional units, if appropriate, and provide SI units parenthetically in the text and give conversion factors in table footnotes and figure legends. Units should not be pluralized. Useful websites are:

SI conversion website: http://www.ex.ac.uk/cimt/dictunit/dictunit.htm

Clinical SI conversions: http://www.unc.edu/~rowlett/units/scales/clinical_data.html

Clinical SI conversions: http://dwjay.tripod.com/conversion.html

ABBREVIATIONS. Use only standard abbreviations. Table 2 is an abridged list of abbreviations that may be used without definition in Journal of Nutrition articles. Other standard abbreviations are listed in Scientific Style and Format (1).

Full gene names are not required for tables and figures in which a database identifier number is given. A full citation to the database should be in Literature Cited and the reference number provided in the figure legend or table footnote. If the genes are listed in online supporting material (supplemental tables and figures), the citation can be given as a table footnote or in the figure legend [e.g. National Center for Biotechnology Information (NCBI) Entrez Gene (http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene) or Unigene (http://www.ncbi.nlm.nih.gov/unigene).]

For genes that are discussed in some detail in the text, either the full name of the gene or a word description of the functional activity that helps identify it should be provided for clarity and the abbreviation should be included in the manuscript's abbreviation footnote.

If there are three or more abbreviations defined in the text, define each the first time it is used in the text and prepare an abbreviation footnote. The footnote should be associated with the first abbreviated term in the text and should be an alphabetized listing of all author-defined abbreviations and their definitions. Abbreviations should not be followed by a period and should not be pluralized (e.g. AA should represent both "amino acid" and "amino acids"). Use the verb (e.g."is" or "are") that is consistent with the context in which the abbreviation is used in the sentence. Units and statistical terms also should not be followed by a period or pluralized. Use the standard abbreviations for SI prefixes found in Young (9) and in Table 3 and those for units of measure in Table 4. Abbreviations used only in tables and figures must be separately defined in the footnotes or legend for each table or figure. Abbreviations that are in the abbreviation footnote should not be redefined in table footnotes or figure legends.

NOMENCLATURE. Chemical and biochemical terms and abbreviations and identification of enzymes must conform to the recommended usage of the International Union of Biochemistry and Molecular Biology (10). Names for vitamins, related compounds, and abbreviations for amino acids should follow the ASN nomenclature policy (11,12).