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© 2007 American Society for Nutrition J. Nutr. 137:964-972, April 2007

Nutrition and Disease

Soy Isoflavones Exert Differential Effects on Androgen Responsive Genes in LNCaP Human Prostate Cancer Cells1

Lori Rice2,*, Renita Handayani3, Yuehua Cui8, Theresa Medrano3, Von Samedi3, Henry Baker4, Nancy J. Szabo5, Charles J. Rosser6, Steve Goodison7 and Kathleen T. Shiverick3

Departments of 2 Radiation Oncology, 3 Pharmacology and Therapeutics, 5 Molecular Genetics and Microbiology, and 6 Urology, College of Medicine; 4 Department of Statistics, College of Liberal Arts and Sciences; and 7 Analytical Toxicology Core Laboratory, College of Veterinary Medicine; University of Florida, Gainesville, FL 32610 and 8 Department of Pathology, University of Florida, Jacksonville, FL 32209

* To whom correspondence should be addressed. E-mail: lrice{at}ufl.edu.


   ABSTRACT
TOP
 ABSTRACT
Introduction
 Materials and Methods
 Results
 Discussion
 LITERATURE CITED
 
The high consumption of soy isoflavones in Asian diets has been correlated to a lower incidence of clinically important cases of prostate cancer. This study characterized the effects of a soy-derived isoflavone concentrate (ISF) on growth and gene expression profiles in the LNCaP, an androgen-sensitive human prostate cancer cell line. ISF caused a dose-dependent decrease in viability (P < 0.05) and DNA synthesis (P < 0.01), as well as an accumulation of cells in G2/M, and G0/G1 phases of the cell cycle compared with controls. Using Affymetrix oligonucleotide DNA microarrays (U133A), we determined that ISF upregulated 80 genes and downregulated 33 genes (P < 0.05) involving androgen-regulated genes and pathways controlling cell cycle, metabolism, and intracellular trafficking. Changes in the expression of the genes of interest, identified by microarrays, were validated by Western immunoblot, Northern blot, and luciferase reporter assays. Prostate-specific antigen, homeobox protein NKX3, and cyclin B mRNA were significantly reduced, whereas mRNA was significantly upregulated for p21CIP1, a major cell cycle inhibitory protein, and fatty acid and cholesterol synthesis pathway genes. ISF also significantly increased cyclin-dependent kinase inhibitor p27KIP1 and FOXO3A/FKHRL1, a forkhead transcription factor. A differential pattern of androgen-regulated genes was apparent with genes involved in prostate cancer progression being downregulated by ISF, whereas metabolism genes were upregulated. In summary, we found that ISF inhibits the growth of LNCaP cells through the modulation of cell cycle progression and the differential expression of androgen-regulated genes. Thus, ISF treatment serves to identify new therapeutic targets designed to prevent proliferation of malignant prostate cells.


   Introduction
TOP
 ABSTRACT
 Introduction
Materials and Methods
 Results
 Discussion
 LITERATURE CITED
 
An estimated 234,000 men will be diagnosed with prostate cancer in the United States in 2006, and ~27,000 will result in death (1). Epidemiological studies have correlated a relatively low incidence of prostate and other cancers with populations having a high dietary intake of soy products (2–3). These findings have generated an interest in the chemotherapeutic effects of isoflavones, a class of phytoestrogens found in high concentrations in soy and other legumes (4).

The antiproliferative effects of genistein, the predominant isoflavone in soy, on various types of cancers, including prostate cancer, have been well documented (5). Genistein can induce apoptosis and inhibit the activation of the antiapoptotic protection factor, NF-{kappa}ß, in prostate cancer cells (6). In addition, genistein inhibits growth in prostate and other cancer cells by the upregulation of p21cip1/waf1 and a concomitant decrease in cyclin B, resulting in G2/M arrest (7). Studies have shown that higher levels of p21 expression are associated with a more favorable prognosis for patients with recurrent prostate cancer after radiation therapy (8). Thus, identifying molecular targets may further the development of new therapeutic strategies.

The advent of cDNA microarray technology allows researchers to profile virtually the entire expressed genome of specific cell types as well as to investigate the effects of potentially useful antiproliferative agents on thousands of genes simultaneously. Using prostate-specific filter arrays, we showed that biochanin A, a red clover-derived isoflavone, inhibits the growth of the prostate cancer cell line, LNCaP, in vitro and in mouse xenografts (9). In this study, because a growing number of cancer patients self-medicate with nutritional supplements (10), with >15% of prostate cancer patients surveyed taking a soy product after diagnosis (11), we used microarray chips to investigate the effects of a commercially available dietary soy-derived isoflavone (ISF)9 supplement (NovaSoy) on LNCaP cells.


   Materials and Methods
TOP
 ABSTRACT
 Introduction
 Materials and Methods
Results
 Discussion
 LITERATURE CITED
 
    Cell culture and treatment conditions. The human prostate carcinoma cell line, LNCaP, was obtained from American Type Cell Culture Collection, cultured in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 100 kU/L penicillin-streptomycin, and 2 mmol/L L-glutamine, and maintained in a humidified 5% CO2 incubator at 37°C. A tissue culture–compatible form of NovaSoy, containing 49% ISF by weight, was obtained from Archer Daniels Midland Company (12) and dissolved in 0.1% dimethyl sulfoxide (DMSO). NovaSoy approximates the natural composition of ISF in soybeans and is low in protein (8.5%) and fat (0.42%). LNCaP cells were treated with NovaSoy at indicated concentrations, or with 0.1% DMSO vehicle alone, for 48 h.

    Isoflavone analysis. The amounts of free and conjugated glucosides of genistein and daidzein in NovaSoy-treated medium were determined using reversed-phase HPLC with UV and mass spectral detection in series (13–14), as described in detail previously (15). This allowed us to determine the extent to which LNCaP cells hydrolyze the glucoside forms of ISF in NovaSoy to the metabolically active aglycone forms.

    Cell viability. LNCaP cell viability was determined by the ability of ISF-treated and control cells to exclude 0.1% trypan blue (Sigma-Aldrich).

    [3H]-Thymidine incorporation assay. Cells were treated with 0.1–200 mg/L ISF or DMSO vehicle for 48 h. [3H]-Thymidine (4 µCi/well) was added to the medium and pulsed for 16 h. Cells were collected on glass fiber filters using a Brandel cell harvester and the amount of incorporated [3H]-thymidine was determined by liquid scintillation counting.

    Flow cytometry. Isolation and staining of cell nuclei were performed using the Cycle Test Plus DNA reagent kit (Becton Dickinson) according to the manufacturer's protocol. The cells were then subjected to flow cytometric analysis on FACSort (Becton Dickinson) and analyzed by CELL Fit software program. MODFit software was used to quantify nucleic DNA content and extrapolate cell cycle phase distribution based on ratios of mean fluorescence.

    Western immunoblots. Western immunoblotting was performed as described previously (11,15), using primary antibodies to human p21CIP1/WAF1, cyclin B (BD Transduction Laboratories), p27KIP1, prostate specific antigen (PSA) (DakoCytomation), human GST-A1 (Oxford Biomedical Research), or FKHRL1 (United Biochemicals), and secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG secondary antibodies (Bio-Rad Laboratories). Subsequently, membranes were incubated with anti-human actin mouse monoclonal antibody (Oncogene Research Products) to verify equal loading and transfer efficiency. Specific proteins were detected using enhanced chemiluminescent detection system (Amersham Pharmacia Biotech).

    Northern blots. Northern blotting was performed, as described previously, on samples of total RNA isolated by the guanidine thiocyanate method (16). Membranes were hybridized with 32P-labeled probe prepared from a p21 cDNA plasmid, generously provided by Dr. Bert Vogelstein, Johns Hopkins Oncology center, Baltimore, MD (17) and exposed to radiographic film for detection and quantification of mRNA signals using Scion Image software (Scion). The signals were normalized for the total RNA loading and transfer efficiency with ß-actin mRNA.

    Transfection. LNCaP cells were transfected with a plasmid containing wild-type p21 promoter (p21p)/luciferase reporter obtained from Dr. Vogelstein (17) and cotransfected with pRL-TK vector (Promega) using Lipofectamine 2000 (Invitrogen Life Technologies) according to supplier instructions. Cells were then exposed to 150 mg/L ISF or DMSO vehicle for 48 h and subsequently assayed with the Dual Luciferase Assay System (Promega). Luciferase activity was measured using a MonoLight Illuminator-3010 (Pharmingen). The activity of each assay was normalized to the activity of the internal control reporter (pRL-TK) to correct for differences in transfection efficiency.

    Statistical analysis of biological assays. Experiments were repeated 3 times and all treatments were expressed relative to the control, which was set at 100%. Values from 3 experiments were presented as means ± SEM. Data were analyzed by ANOVA and Tukey's post hoc test for pairwise comparisons. Statistical analyses were performed using Microsoft Excel with Analyze-It add-in software. Differences were considered significant at P ≤ 0.05.

    Gene expression profiling using oligonucleotide microarray chips. The expressed genomes of ISF-treated and control cells were analyzed using Affymetrix Human Genome U133A GeneChip microarrays, according to manufacturer's protocol. Fluorescence intensity of GeneChips transcripts and present or absent calls were calculated using Affymetrix Microarray Suite 5.0 (MAS 5.0). Hierarchical clustering was performed using DNA-Chip Analyzer (dChip) software (18), as described previously (15). Normalized expression intensities were then visualized using colorimetric matrices with red colors indicating relative overexpression and green colors indicating relative underexpression for a given probe set. For genes of interest, average-linkage hierarchical clustering of the data were applied using Cluster and the results displayed using TreeView (19).

Genes were determined to have altered expression levels in ISF-treated samples compared with DMSO-treated samples based on the following criteria: 1) P ≤0.05 and 2) 1.4-fold or greater difference between the means of the 2 groups, using the lower bound of the 90% CI (20). The reliability of the comparison criteria was assessed by checking the false discovery rate via permuting the samples. Differentially expressed genes were then categorized based on their cellular component, biological process, and molecular function using Onto-Express (21).

Associations with gene ontology biological process, molecular function, cellular component groups, and GenMAPP biological pathways were obtained with MAPPFinder, a freely available software tool that colors biological pathways with gene expression data (22). MAPPFinder Z-scores, a statistical measure of significance for gene expression in a given group, were calculated by subtracting the number of genes expected to be randomly changed in a gene ontology term from the observed number of changed genes in that term.


   Results
TOP
 ABSTRACT
 Introduction
 Materials and Methods
 Results
Discussion
 LITERATURE CITED
 
    Effect of ISF on proliferation and cell cycle progression. Based on 3H-thymidine incorporation and trypan blue dye exclusion assays, ISF dose-dependently inhibited both cell viability (P < 0.05) and DNA synthesis (P < 0.01) (Fig. 1). A concentration of 42 mg/L ISF produced a 50% inhibition (IC50) of DNA synthesis. A cytostatic concentration of 150 mg/L, resulting in >90% inhibition, was used in subsequent assays.


Figure 1
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  		Figure 1  Effects of ISF on DNA synthesis and viability of LNCaP cells treated with 0–150 mg/L for 48 h. Data are means ± SEM, n = 3. Superscripts without a common letter differ from treatment groups: *P < 0.05.

 
Flow cytometry was used to determine whether the antiproliferative effects of ISF were associated with alterations in cell cycle phase distribution. ISF treatment for 48 h decreased the percentage of cells in S phase by 74.6% (P < 0.01). Concomitantly, ISF increased the accumulation of cells in G0/G1 and G2/M phases by 8.9 and 82.5%, respectively (P < 0.05). Interestingly, based on a lack of a sub-G0 peak and absence of PARP cleavage (data not shown), there was no indication of apoptosis. These data indicate that ISF has potent effects on LNCaP cell proliferation and cell cycle progression.

    Availability of free isoflavones in culture medium. Analysis by HPLC-MS showed that the ISF stock solution contained 11% total aglycone equivalents of genistein and 9% daidzein total aglygone equivalents by weight. These predominant isoflavones were present in the bound glucoside form (97%) with only 3% as free aglycones.

Following the exposure of LNCaP cells to 150 mg/L ISF for 48 h, virtually all of the daidzein and genistein was in the metabolically active aglycone form at concentrations of 5.6 mg/L (21.8 µmol/L) and 7.7 mg/L (28.5 µmol/L), respectively, indicating there was complete hydrolysis of the glucoside conjugate (Table 1). In addition, the total amount of genistein and daidzein in the culture medium was reduced by 40%, providing evidence that these cells were able to metabolize the ISF in this soy extract.


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  		TABLE 1 Concentrations of genistein and daidzein in LNCaP cell culture medium after 48-h exposure to ISF1

 
    Effect of ISF on gene expression profiles, including changes in androgen-regulated genes. Gene expression profiles were evaluated using DNA microarray analyses to identify pathways involved in growth inhibition. Samples were 1st analyzed by hierarchical clustering using a filtered subset of 348 variably expressed genes. A total of 113 known genes were significantly altered, with 80 genes upregulated and 33 genes downregulated (Fig. 2A). When permuting samples 50 times, the median false discovery rate was 1.1%, indicating that the comparison criteria were reasonable.


Figure 2
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  		Figure 2  Hierarchical cluster images of LNCaP genes altered by 150 g/L ISF. The treatment groups are DMSO, cells treated with 0.1% DMSO vehicle alone, and ISF-150, with 150 mg/L soy ISF extract. Column labels represent chip replicates. Cluster image of 113 differentially expressed genes (A). Of these, 80 were upregulated and 33 were downregulated. Cluster image, generated by Cluster and TreeView, of a subset of the differentially expressed genes that are directly or indirectly regulated by androgens (B). Cluster image showing coordinated regulation of a subset of genes in fatty acid and cholesterol synthesis pathways that includes the androgen-regulated genes from panel B (C). Expression changes in genes of the cholesterol biosynthesis pathway (D). Using gene-association files from the Gene Ontology Consortium, MAPPFinder assigns genes in the dataset to numerous GO terms and creates functional MAPP if significant (i.e., Z-score > 2.0).

The color scale is obtained by normalization so that the magnitude (sum of the squares of the values) of a row vector = 1; red indicates relative overexpression and green relative underexpression for a given probe set (A–C). Genes that increased 2 times more than control are shown in red, those that increased less than 2 times are shown in purple (D). Figures reproduced with permission from (22). Lieberman, M. and Mantei, N. Gladstone Institutes.

 
A subset of ISF-regulated genes was identified by a cross-comparison with previously published datasets of androgen-regulated genes in prostate (23–30). Androgen-regulated genes of interest that were altered by ISF treatment are represented by a hierarchical clustering image (Fig. 2A). Table 2 lists the fold-changes and putative functions of 27 ISF-regulated genes that have been directly or indirectly related to androgen regulation.


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  		TABLE 2 Proliferation, cell cycle control, and/or tumor progression of androgen-regulated genes in LNCaP cells exposed to 150 mg/L of a soy isoflavone concentrate compared with vehicle alone1

 
LNCaP is an androgen-sensitive cell line, so it is likely that phytoestrogenic agents, such as ISF, could affect expression of androgen-regulated genes. Of particular interest in this group were the highly downregulated genes kallikrein-2 (KLK2) and kallikrein-3 (KLK3) that encode PSA, a major marker for prostate cancer proliferation (23). In addition, ISF markedly decrease NKX3.1, a homeobox protein, and MAF, a v-maf oncogene homolog (31). An unexpected finding was that ISF upregulated a number of genes involved in metabolic pathways reported to be androgen regulated. This group included malic enzyme 1 (ME1), stearoyl-CoA desaturase (SCD), isopentenyl-diphosphate delta isomerase (IDI1), and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1). These data are consistent with reports of androgen regulation of several lipogenic enzymes (29–32). In this regard, however, a divergent pattern of androgen-regulated genes apparent with the primary genes involved in prostate cancer progression were downregulated by ISF, whereas the metabolism genes were upregulated (Table 2). However, several important androgen-related genes were not altered by ISF, including the androgen receptor, prostate androgen-related transcript-1 (Part-1), and alpha-methyl CoA racemase (AMACR).

Table 3 lists a number of genes involved in metabolism, apoptosis/stress responses, molecular function, and cell cycle regulation. Among key upregulated genes were FOXO3A, a forkhead transcription factor involved in the regulation of proapoptotic genes (31) and CDKN1A, the gene that encodes p21cip1 protein, a major cyclin-dependent kinase inhibitor. Similarly, cyclin B, which is essential for G2/M cell cycle progression, was downregulated. Overall, changes induced by ISF in cell cycle regulatory genes support our cell viability and flow cytometry data.


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  		TABLE 3 Ontological classification of other genes significantly altered by an isoflavone concentrate in LNCaP cells, as described in Table 2

 
An unexpected finding was that ISF upregulated a number of genes involved in lipid metabolism pathways, including fatty acid desaturase-2 (FADS2), lanosterol synthase (LSS), and fatty acid-CoA synthetase long-chain family member 1 (FACL2/ACSL1). Figure 2A includes a hierarchical clustering image showing coordinated regulation of a subset of genes in fatty acid and cholesterol synthesis pathways that includes the androgen-regulated genes from Figure 2B. It is striking that they were nearly all upregulated by ISF, suggesting that ISF may be acting to alter expression of sterol-regulatory binding proteins (SREBP), lipogenic transcription factors that regulate cellular lipid homeostasis. However, SREBPs-1c and 2 were not present on our array list. MAPPFinder analysis identified several biological processes influenced by ISF, including cell cycle, G-protein signaling, and apoptosis. Of particular interest was the validation of microarray data showing the upregulation of cholesterol synthesis genes by ISF (Fig. 2B). Seven of 14 genes in the cholesterol synthesis MAPP were significantly increased.

    ISF upregulates p21cip1 mRNA and protein via transcriptional and translational activation. Microarray data indicated that ISF upregulated CDKN1A, the gene that encodes the p21cip1 protein. Northern blot analysis also showed that ISF increased the expression of CDKN1A mRNA by 400% (Fig. 3A) and protein by 60% (Fig. 3B), (P < 0.05), indicating regulation at both the transcriptional and translational levels.


Figure 3
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  		Figure 3  Validation of microarray data for p21CIP1 protein and mRNA expression in LNCaP cells treated with 150 mg/L ISF. Northern blot of p21CIP1 mRNA (A). Western immunoblot of p21CIP1 protein expression (B). Representative blots are shown above histograms. Data from Northern and Western blots were normalized to ß-actin signals and to control cells treated with DMSO vehicle alone. Activation of a p21 promoter-luciferase construct by ISF treatment in cells transfected with a wild-type p21 promoter/luciferase reporter (C). Values are means ± SEM, n = 3. Asterisks indicate different from DMSO control: *P < 0.05, **P < 0.003.

 
It was of further interest to determine whether the ISF effect on p21cip1 mRNA level was due to increased transcriptional regulation of p21 or other mechanisms such as message stability. LNCaP cells, transfected with a p21cip1 promoter/luciferase reporter construct and then analyzed by dual luciferase assay following treatment with ISF or DMSO vehicle alone, showed that ISF enhanced transcription of the p21cip1luciferase reporter by 175% (P < 0.003) (Fig. 3C), which suggests direct transcriptional regulation through the gene promoter. To our knowledge, this is the first reported evidence of direct transcriptional regulation of the p21 promoter by an isoflavone mixture.

    Soy ISF downregulate PSA mRNA and protein expression. Differential expression of selected genes identified by microarray analyses was validated by independent methods. Microarray data indicated that ISF significantly downregulated the level of PSA mRNA by 86%. PSA is an androgen-regulated biological marker positively associated with prostate cell number. Western immunoblots confirmed that protein levels were also decreased by 96% (P < 0.01) (Fig. 4A).


Figure 4
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  		Figure 4  Validation of microarray data for PSA (A), cyclin b (B), p27 (C), and FOXO3A (D) expression by Western immunoblotting in LNCaP cells treated with 150 mg/L soy isoflavone extract. ß-Actin expression was used to confirm equal loading and transfer efficiency across the lanes (representative blot shown in panel A). Data were normalized to band intensity of control cells treated with DMSO vehicle alone and are shown as means ± SEM, n = 3. Asterisks indicate different from DMSO control: *P = 0.019, **P = 0.007.

 
    ISF affects expression of cyclin B, p27, and FOXO3A, but not glutathione S-transferases. Microarray data indicated that ISF significantly decreased the abundance of cyclin B2 mRNA, a regulator of G2/M progression, by 60% compared with control values. Western immunoblot analysis confirmed that ISF decreased cyclin B protein by 54% (P = 0.007) (Fig. 4B). In contrast, ISF increased protein levels of p27KIP1, a 2nd major cyclin-dependent kinase inhibitor (P = 0.019) (Fig. 4C). An unexpected finding of a novel gene upregulated by ISF, as validated by Western immunoblotting (Fig. 4D), was FOXO3A/FKHRL1, a forkhead transcription factor involved in the regulation of proapoptotic genes, including p27 (31).

Decreased expression of glutathione S-transferases (GST), a family of proteins with antioxidant free-radical scavenging properties has been associated with disease progression in prostate cancer patients (32). Microarray data showed that GST-A1, but not GST-M1 or GST-P1, mRNA was expressed in LNCaP cells and that the level of GST-A1 transcripts was not affected by ISF treatment. Western immunoblot analysis confirmed that GST-M1 and GST-P1 were below the assay level of detection, and that ISF did not alter GST-A1 expression at the protein level (data not shown).


   Discussion
TOP
 ABSTRACT
 Introduction
 Materials and Methods
 Results
 Discussion
LITERATURE CITED
 
This study shows that treatment of LNCaP cells with cytostatic doses of a soy isoflavone concentrate alters global gene expression. The development of microarray technology to analyze multiple genes simultaneously allows the evaluation of changes in differentially regulated genes along functional pathways as well as the identification potential therapeutic targets. The present study utilized Affymetrix GeneChips with the capacity to interrogate over 17,000 human genes to determine the effect of a soy extract on the gene expression profiles of LNCaP cells.

The isoflavones in NovaSoy are predominantly in the bound glucoside form. However, extensive hydrolysis by LNCaP cells during the 48-h incubation period resulted in virtually all of the major ISF, daidzein and genistein, being converted to their more metabolically active "free" aglycone forms, with a 40% reduction in the total amount of these ISF.

During these studies, LNCaP cells were exposed to an ISF supplement containing 59 µmol/L genistein. As little as 15 µmol/L genistein has been shown to affect a 40–60% decrease in PC-3 DNA synthesis, cell viability, and colony formation (33). This agrees with other studies showing that 2.6–79 µmol/L genistein was required to produce a 50% growth inhibition in most cancer cell lines (34). Although 13 µmol/L is considered the upper limit of genistein in the serum of people consuming a high soy diet (34–35), prostate cancer patients given high doses (300–600 mg) of NovaSoy had circulating genistein concentrations of up to 27 µmol/L, with no evidence of genotoxicity (36). Mice with orthotopically implanted LNCaP xenografts that were fed 4 mg/d total genistein equivalents, either as genistein or as a component of an ISF concentrate similar to the one used in these studies, had similar serum genistein levels of 1.6 µmol/L and 1.8 µmol/L, respectively (37). This was sufficient to cause a 70% reduction in tumor growth compared with controls. However, the isoflavone diet resulted in significantly greater induction of apopotosis, enhancement of p53 expression, and inhibition of metastatic activity, compared with genistein alone. Although genistein, the predominant isoflavone in soy, is by itself a potent antiproliferative agent against prostate cancer cells, the authors conclude that the concentrate was even more effective. These studies indicate that isoflavones can be consumed in adequate quantities to exert biological effects. At the same time, it is recognized that higher doses of isoflavones are required in cell culture models to attain the same degree of growth inhibition of prostate cancer cells as seen with xenograft tumors in mice fed an ISF-supplemented diet (12,34,37). Therefore, the multiple biological processes influenced by isoflavones may have a greater impact in the microenvironment of solid tumors.

Our data demonstrate that ISF caused a dose-dependent inhibition of cell viability and DNA synthesis in LNCaP cells. Furthermore, ISF at 150 mg/L was found to inhibit DNA synthesis by 91% and induce accumulation of cells in G0/G1 and G2/M phases of the cell cycle. Microarray analysis identified 113 genes were significantly altered by ISF treatment, with 80 genes upregulated and 33 downregulated. The changes in expression of genes such as p21 (upregulated) and cyclin B2 and kallikrein/PSA (downregulated) suggest a role in ISF-induced cytostasis. Genes involved in fatty acid metabolism also appeared prominently in the list of ISF-regulated genes.

LNCaP cells are androgen sensitive with a mutated but functional androgen receptor (AR). Antiandrogens that suppress androgen production, or competitively inhibit ligand binding, are used clinically to slow the growth of malignant prostate cells that continue to retain expression of the androgen receptor. Therefore, downregulation of the AR or AR-regulated genes also may be useful in reducing androgen-stimulated proliferation of tumor cells. Our dataset agrees with those of other investigators who found that, although isoflavones regulate androgen-responsive genes, the AR itself may or may not have altered expression. These agents can work to affect activity of the AR via transacting factors. Several datasets comparing the transcriptomes of androgen-supplemented or deprived LNCaP cells, generated using techniques such as SAGE (24–25), oligonucleotide/cDNA arrays (23–24,26), tissue arrays (38), or proteomics (39–40) have been published. Therefore, we were able to identify 46 differentially expressed genes from our data that have been determined to be directly or indirectly regulated by androgens.

Interestingly, although several of the genes listed in Table 2 were also reportedly altered in LNCaP cells by PC-SPES, an herbal supplement known to downregulate the AR (41), some AR-regulated genes responded differently to these 2 extracts. ISF and PC-SPES both inhibited genes with proliferative functions such as KLK/PSA, a serine protease marker for prostate growth (23); beta tubulin, often a target of anticancer drugs such as paclitaxel and vincristine (42); and NKX3.1, a prostate-specific transcription factor. However, there are conflicting reports in the literature regarding changes in NKX3.1 expression as related to tumor progression (38). MAF, a member of a family of differentiation response proteins, is sometimes overexpressed in cancer (23). MAF was downregulated by ISF but not by PC-SPES.

Both ISF and PC-SPES upregulated the cdk-inhibitor CDKN1A/p21 in LNCaP cells. However, ISF upregulated DnaJ, whereas there was no change in PC-SPES-treated cells. Selenium-binding protein 1 (SELENBP1) was upregulated by ISF but decreased by PC-SPES. The ongoing Selenium and Vitamin E Cancer Prevention Trial (SELECT) is based on the theory that selenium may have potent anticancer effects by protecting healthy cells from oxidative damage (43).

Many androgen-regulated genes are involved in metabolism, which may explain the growth-promoting effect of steroids on prostate cell lines with a functioning AR. Most of the genes involved in the biosynthesis of fatty acids and cholesterol are upregulated, such as malic enzyme (ME), fatty acid CoA ligase, long chain 2 (FACL2), stearoyl-CoA desaturase (SCD), and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) (Table 3). Many are known to be under transcriptional control of the sterol regulatory element-binding proteins SREBP-1 and SREBP-2. SREBP are major activators and regulators of fatty acid and cholesterol biosynthesis and link these pathways to nutritional status (44). Furthermore, ISF have significantly upregulated the expression of isopentenyl-diphosphate isomerase (IDI1) whose protein encodes an enzyme involved in sterol synthesis (45). In contrast, UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1), an enzyme involved in the synthesis of complex carbohydrates, was downregulated. Lipid metabolism genes such as acyl CoA synthase, HMG CoA synthase, and reductase are known to be transcriptionally regulated through androgen response elements in the promoter regions (21,38,41). Cytostatic doses of ISF, which can have weak estrogenic effects, may trigger a damage or stress response that mimics the metabolic effects of androgens.

In agreement with our microarray data, Western immunoblot analysis of the expression of GST in LNCaP cells revealed the presence of GST-A1, which was not altered by ISF treatment. GST-M1 and GST-P1 expressions were below the level of detection. Although GST-P1 expression is abundant in most tumors, it is often lost in prostate cancer and has been implicated in disease progression (46).

This study demonstrated that ISF treatment significantly altered genes involved in multiple cellular processes, including proliferation, cell cycle regulation, cholesterol synthesis, and lipid metabolism. More than 40 androgen-regulated genes were affected. Although the effects of isoflavones on reducing the risk of prostate cancer, or providing benefits to those already diagnosed with this disease, have yet to be unequivocally determined, reports of the low incidence of prostate cancer in populations with high soy diets have resulted in many men taking soy-based supplements (47). It is not yet known how high levels of circulating isoflavones may affect treatments such as androgen ablation or brachytherapy or the incidence of recurrence. Thus, further studies investigating isoflavone-targeted genes may open new avenues for chemoprevention or therapy for prostate cancer.


   ACKNOWLEDGMENTS
 
The authors thank Dr. Burt Vogelstein (Johns Hopkins Oncology Center, Baltimore, MD) for plasmids containing p21 CIP1 cDNA.


   FOOTNOTES
 
1 This work was supported by grant CA91231 from the National Cancer Institute, NIH, and P42 ES07375 from the National Institute of Environmental Health Sciences. Back

9 Abbreviations used: AR, androgen receptor; DMSO, dimethyl sulfoxide; ISF, soy isoflavone extract; PSA, prostate specific antigen. Back

Manuscript received 30 April 2006. Initial review completed 5 June 2006. Revision accepted 17 January 2007.


   LITERATURE CITED
TOP
 ABSTRACT
 Introduction
 Materials and Methods
 Results
 Discussion
 LITERATURE CITED
 

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