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Figure 2. Relationship of histochemical score with kidney calcium concentration in female Sprague-Dawley rats fed diets containing different concentrations of Ca and P for 16 wk (see text for details). Values are means ± SD, n = 8, 15, 8, 5, 4 for histochemical scores of 0, +, ++, +++ and ++++, respectively. Histochemical score is based on the number of granules seen in 1 transverse and 1 longitudinal kidney section (von Kossa stain): 0 = 0 granules seen, + = 1–25, ++ = 26–75, +++ = 76–150, ++++ = >150 granules. Spearman Rank Order correlation coefficient for the relationship of histochemical score with kidney calcium r = 0.87 (P < 0.00001).

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