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Departments of Animal Science and * Food Science, North Carolina State University, Raleigh, NC 27695-7621
3To whom correspondence should be addressed. E-mail: jack_odle{at}ncsu.edu.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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9 desaturase activity. The iodine value of belly fat from pigs consuming tallow and CLA combined was reduced to 62.0 from an initial value of 70.4. CLA supplementation also increased belly weights (P < 0.05). CLA did not affect longissimus muscle area, backfat depth and the percentage of fat-free lean (P > 0.10), but it increased the subjective intramuscular fat score by 18.8% (P < 0.01). In conclusion, CLA enrichment of pork products may be enhanced when combined with additional supplemental dietary fat, and together with tallow can be used to increase the saturated fatty acid content of pork.
KEY WORDS: • conjugated linoleic acid • fatty acid composition • pork • supplemental fat • swine
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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). Both classical studies and epidemiologic evidence were reviewed and it was concluded that (n-6) PUFA lower cholesterol and are protective against coronary heart disease and atherosclerosis (1
). The swine industry has responded to these changing consumer preferences by developing leaner genotype pigs and by supplementing swine diets with unsaturated fat sources (2
). However, increases in linoleic acid and other PUFA are undesirable to pork processors because increased PUFA content results in soft belly fat, which leads to poor bacon slicing and may result in increased rancidity. Therefore, we evaluated the effects of alternative supplemental dietary fat sources for swine, including hydrogenated fat, tallow and conjugated linoleic acid (CLA) (3
,4
).
Development of pork products enriched with CLA may offset the negative effect of diets containing saturated fatty acids because several studies have shown that adding 
1 g/100 g CLA to the diet can provide protection against cancer comparable with that provided by 18 g/100 g fish oil (5
,6
), whereas others have observed antiatherogenic effects (7
). In addition, supplementation of swine diets with CLA has reduced backfat depth and improved belly firmness (8
–12
). Because CLA has the potential to alter gene expression of key lipogenic enzymes (13
,14
), including stearoyl-CoA desaturase (15
), and supplemental fat can be directly deposited in swine adipose tissue, their combination may result in additive effects on pork fat composition and quality. Therefore, we tested the hypothesis that dietary CLA would increase the saturated/unsaturated ratio of pork fat and thus positively affect belly firmness. We further hypothesized that supplementation of a more saturated fat source [tallow; iodine value (IV) = 47] with CLA would further improve belly firmness beyond that occurring if CLA were combined with a more unsaturated fat source (yellow grease; IV = 83).
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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All animal procedures were approved by the Institutional Animal Care and Use Committee of North Carolina State University. Lean genotype gilts (n = 144; mean weight, 49.3 kg) were delivered to the North Carolina Swine Evaluation Station from a large, integrated, North Carolina Pork Producer and were randomly blocked into one of two slaughter groups. Pigs were then randomly assigned to one of six treatments according to a 3 x 2 factorial design (24 pigs per treatment; 3 pigs/pen). Diets (Table 1
) were formulated to exceed NRC (16
) nutrient requirements for finishing swine and included three sources of supplemental fat and two sources of linoleic acid (LA) as follows: 1) no supplemental fat + 1 g/100 g LA; 2) no supplemental fat + 1 g/100 g CLA; 3) 4 g/100 g yellow grease + 1 g/100 g LA; 4) 4 g/100 g yellow grease + 1 g/100 g CLA; 5) 4 g/100 g tallow + 1 g/100 g LA; and 6) 4 g/100 g tallow + 1 g/100 g CLA.
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60% conjugated isomers as shown in Table 2
. This product was chemically prepared from sunflower oil and was supplemented as a free fatty acid. Corn oil was chosen as the control because the LA content was also 60% (Table 2)
. The yellow grease and tallow had IV of 82.8 and 47.2, respectively. To ensure oxidative stability, the supplemented fats were stabilized with 0.1% ethoxyquin, and the interval between diet mixing and consumption by pigs was minimized ( 1 mo). Dietary treatments (Table 1)
were initiated after a 1-wk acclimation period. Pigs were divided into two groups by weight (n = 72) with one group beginning treatment and being slaughtered 1 wk before the second group. Pigs consumed food and water ad libitum for 6 wk until they reached an average slaughter weight of 113 kg. They were weighed before initiation of treatments and then every 2 wk until slaughter. Feed consumption was recorded throughout the study. Backfat depth and longissimus muscle area were measured by real-time ultrasound (ALOKA 500V; Animal Ultrasound Services, Ithaca, NY) immediately before slaughter.
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Hot carcass weight was determined at slaughter. Carcasses were chilled for 24 h at which time a 2.5-cm loin chop was removed between the 9th and 10th ribs. After allowing a minimum of 20 min bloom time, each chop was evaluated for color, ultimate pH and marbling score. The longissimus muscle chop was measured in triplicate (middle, medial and lateral) and mean values were calculated for color lightness (L*), redness (a*) and yellowness (b*) using a Minolta Chromometer 200 (Minolta, Ramsey, NJ). The chromometer was set to D65 illuminant, a 2° standard observer, using an 8-mm optical port with glass insert, and calibrated with Minolta white standard color plate. A visual color score was also determined on a scale from 1 to 6 (1 = pale, 6 = very dark) using plastic Japanese color standards (17
). Japanese color standards are closely related to the Minolta L* value but the scales are in the opposite direction. A lower Minolta L* value indicates a darker color. Marbling scores were determined subjectively using a visual scale (1 = 1% intramuscular fat, 6 = 6%) (17
). On the same sample, ultimate pH (24 h postslaughter) was measured using an Engold electrode and a K21 pH meter (NWK Binar, Landsberg, Germany).
The percentage of fat-free lean was estimated using a prediction equation for unribbed carcasses using the 10th rib loin muscle area and fat depth derived from real-time ultrasound and hot carcass weight (17
). Drip loss (mg) was estimated by placing a preweighed Whatman #1 filter paper on a longissimus muscle section removed from between the 9th and 10th rib for 1 min. The filter paper was then reweighed and purge loss determined. The percentage of drip loss was estimated by the following equation: 48-h drip loss = -0.1 + (0.06) x (mg fluid) ± 0.09; R2 = 0.90 (18
). Initial backfat tissue cores were taken from each pig at the 10th rib, 5 cm from the backbone and 2.5 cm deep as described previously (4
). The initial content of fatty acids of the subcutaneous fat was as follows (g/100 g): 16:0 = 19.20; 16:1 = 5.84; 18:0 = 10.72; 18:1 trans-9 = 0.83; 18:1 cis-9 = 39.83; 18:2 cis-9, cis12 = 17.25. Belly fat cores were removed from the shoulder end of the belly 24 h after slaughter (n = 8/treatment). Fat samples were placed in N2 gas at the time of collection, stored at -80°C and analysis was completed within 6 mo of collection. Lipids were extracted from longissimus muscle samples (n = 8/treatment) in duplicate before fatty acid analysis (19
). Lipids were isolated from adipose tissue in duplicate and fatty acid composition was determined by gas-liquid chromatography as described previously (4
). The IV was calculated from fatty acid composition data using the following equation (20
): IV = 16:1 (0.95) + 18:1 (0.86) + 18:2 (1.732) + 18:3 (2.616) + 20:1 (0.785) + 22:1 (0.723). The IV represents the grams of iodine bound per 100 g of fat.
Belly processing.
A subset of bellies (n = 48; 8/treatment) were collected, squared and processed in a commercial facility. Bellies were weighed [fresh weight (wt)], pumped for a 20% increase in weight with a cure containing salt, sodium nitrite (6.25%), sodium erythorbate (2.5%), sugar, flavorings, FD & C Red #3 (0.00022%) and not > 1% sodium carbonate. After pumping, bellies were weighed (pumped wt) and then smoked for 24 h. Belly weights were recorded after smoking (smoked wt) to determine yield [(smoked belly wt/fresh belly wt) x 100].
Statistical analysis.
Data were analyzed using the General Linear Models procedure of SAS (SAS Institute, Cary, NC). Least-squares treatment means were obtained assuming fixed models that included the effects of group, fat supplementation, LA source and fat supplementation x LA source. The df were further partitioned into contrasts for the effects of supplemental fat source (yellow grease vs. tallow) and supplemental fat level (0 vs. 4 g/100 g). Differences were considered significant at P < 0.05.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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Feed consumption throughout the 47-d experiment was not affected by fat supplementation or addition of CLA to the diets (P > 0.10) and averaged 2.26 ± 0.07 kg/d. Average daily gain during the 47-d experiment was not affected by CLA consumption (0.87 ± 0.03 kg/d). Efficiency of gain (i.e., kg gain/kg feed intake) was improved by supplemental fat (0.37 vs. 0.40 ± 0.01; P < 0.01). In addition, there was a fat source effect with a 2.6% improvement (P < 0.02) in efficiency of gain in pigs consuming yellow grease compared with those consuming tallow.
Carcass quality.
Backfat depth and longissimus muscle area were not affected by supplemental fat or CLA feeding (Table 3
; P > 0.10). Subjective marbling score was 18.8% greater in longissimus muscle chops from pigs fed CLA than in those fed LA (P < 0.01). However, the estimated percentage of fat-free lean was not different between treatments (P > 0.10).
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CLA supplementation increased the weight percentages of 14:0, 16:0, 18:0 and 18:1 trans-9 and reduced the percentage of 18:1cis-9 and 20:1cis-11 (P < 0.001) in belly fat samples (Table 4
). The percentage of LA (cis-9, cis-2) was 12.5% greater in pigs consuming 4 g/100 g yellow grease (P < 0.001) compared with pigs fed tallow. Both the cis-9, trans-11 and the trans-10, cis-12 isomers of CLA were increased in belly fat from pigs fed CLA, and that increase was even greater (ranging from 56 to 86%) when 4 g/100 g supplemental fat was fed (Fig. 1
, fat source x LA interaction, P < 0.001). Total monounsaturates were reduced in belly fat from pigs consuming CLA (P < 0.001), whereas the total percentage of polyunsaturates was increased by 4 g/100 g supplemental fat (P < 0.05), especially in those pigs consuming yellow grease (P < 0.01). CLA supplementation reduced the ratio of monounsaturates to polyunsaturates [monounsaturated fatty acid (MUFA)/PUFA ratio] (2.50 vs. 2.30 ± 0.05; P < 0.001). Addition of CLA to the diet increased the total amount of saturates, while reducing the U/S fatty acid ratio in the belly fat tissue (P < 0.001). Overall, the IV also was affected by both fat and CLA; addition of CLA reduced belly fat IV by 6.6% (P < 0.001) and the IV of fat from pigs consuming tallow and CLA combined was reduced to 62.0 (Fig. 2
).
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).Supplementation of CLA increased the percentages of 14:0 and 18:0 (P < 0.01) and decreased the percentages of 18:1 cis-9, 18:2 cis-9, cis-12 (P < 0.01) and 18:3 cis-9, cis-12, cis-15 (P < 0.05). Again, the cis-9, trans-11 and trans-10, cis-12 isomers of CLA were increased by CLA feeding, especially in pigs fed 4 g/100 g supplemental fat (from 72 to 92%, Fig. 1
; fat supplementation by LA interaction, P < 0.01). Both the total monounsaturates and the MUFA/PUFA ratio were reduced by CLA supplementation (P < 0.01). Feeding 4 g/100 g yellow grease increased total polyunsaturates in fat samples from longissimus muscle chops (P < 0.01; Table 5
). The total saturates increased and the U/S fatty acid ratio decreased in the intramuscular fat with CLA supplementation (P < 0.01), comparable with the response in belly fat.
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The green weight of the cut bellies, before cure injection, was increased 7.2% by CLA supplementation (Table 6
; P < 0.02). The increased belly weights from pigs fed CLA were maintained after pumping (P < 0.02) and smoking (P < 0.08). Supplemental fat source and level did not affect belly weights (P > 0.10).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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). Thus, consumption of diets containing increased (U/S) fat ratios has been recommended by the American Heart Association (1
). Because pork fat can be modified through dietary fat alterations, the swine industry is searching for both effective and economical means of altering the fat composition of swine diets. The fatty acid profile of swine adipose tissue reflects that of the diet, and it is difficult to achieve an ideal (U/S) fatty acid ratio while accommodating the desires of both the consumer and pork processors (21
). Interestingly, recent findings on the effects of consuming a relatively new PUFA, CLA, have shown that this fat has the potential both to affect human health and improve pork processing characteristics in a positive way (5
–11
). Therefore, there are two important issues that form the basis for the modification of pork fat by dietary means. First, CLA has immense potential as a neutraceutical; thus, improved understanding of ways to enrich the CLA content of natural human foods to create a functional food is warranted (22
). Second, bacon consumption is increasing (23
); thus, packers and processors are increasingly concerned about the quality of pork bellies stemming from soft fat depots (24
). In this regard, supplementation of swine diets with CLA also has been shown to increase saturated fat content and thus pork belly firmness (8
,9
). Our study confirms the ease with which dietary fat alterations can be used to modify pork fat composition. In addition, data from our study show, importantly, that the CLA enrichment of pork could be enhanced when CLA was fed with additional supplemental dietary fat.
In response to consumer demands for pork products containing increased PUFA content, the swine industry has increased the number of genetically lean pigs to provide a lower fat product for consumers as well as more efficient production. Scott et al. (25
) showed that genetically lean pigs have decreased endogenous fat synthesis. Thus, the relative proportion of fatty acids in adipose tissue of dietary origin increases in these pigs, making the characteristics of the fatty acids in the diet even more important. A decrease in endogenous fat synthesis partnered with an increased deposition of dietary fatty acids in pigs is beneficial for consumers who are concerned about health because these conditions will result in increased PUFA content in pork products. However, increasing the amount of PUFA in pork is at odds with the fat quality characteristics packers require to process high quality sliced bacon and other processed products. Warnants et al. (21
) have written an excellent review detailing the difficulty of manipulating the pork fatty acid profile with processing technology. Increased PUFA content also leads to a greater amount of lean and fat separation as well as smearing in grinding operations, which results in an undesirable product (26
). For this reason, we chose lean-genotype pigs for this study. Indeed, in our study, the backfat depth was only 14 mm (96.9 kg carcass wt) on average, whereas the swine industry average is 22.9 mm (83.5 kg carcass wt) (27
).
In searching for solutions to improve fat quality, it is important to understand the main factors influencing the quality of backfat. Wood and Enser (28
) showed that stearic acid was positively related to firmness and cohesiveness of fat, whereas LA was negatively related. This is probably because of an increase in membrane fluidity resulting from diets high in LA (29
). Furthermore, lipogenic enzyme activities were increased in adipose tissue of pigs consuming diets with high levels of LA (30
). Therefore, one possible solution to the soft belly fat problem is to supplement diets with a highly saturated fat source. Research in our laboratory has shown that feeding a chemically hydrogenated fat can reduce the PUFA composition of carcass fat and increase pork belly thickness as the saturation of supplemental fat increases (3
).
Another solution yielding improvements in belly firmness has been the supplementation of diets with CLA (8
, 9
). Because of the potential effects of CLA on human health, many researchers have recently evaluated CLA as a swine feed additive. CLA is a term used to describe a mixture of positional and geometric isomers of LA. Ruminant food products such as beef, milk and cheeses are natural sources of CLA. It is produced primarily by biohydrogenation by the ruminant bacteria Butyrivibrio fibrisolvens (31
). However, synthetic sources of CLA are now available. Isomers of CLA have been reported to reduce tumor incidence, reduce body fat and increase body protein (11
,12
,32
). Due to increasing interest of these and other potential effects of CLA on health, researchers have evaluated CLA supplementation in humans using capsules containing < 1 g of CLA each (33
). Although the current number of studies in humans is limited and the data are inconclusive, enrichment of foods with CLA might provide an opportunity for decreasing the risk of cancer and the incidence of obesity or to affect atherosclerosis, all of which have been demonstrated in animal research models.
If research continues to show beneficial effects of CLA consumption in humans and enrichment of CLA isomers in pork could be enhanced, a market for pork as a functional food may exist. We noted an increase in CLA isomers found in both belly fat and longissimus muscle lipid when CLA was supplemented as 1 g/100 g of the diet in this study. Others have also measured increases in both cis-9, trans-11 and trans-10, cis-12 LA isomers with CLA feeding (9
,34
). Interestingly, we measured even greater increases (ranging from 56 to 92%) in CLA content of belly fat and longissimus muscle lipid in pigs consuming 1 g/100 g CLA combined with 4 g/100 g supplemental fat compared with pigs consuming the diets containing 1 g/100 g CLA alone. This increase in CLA content of the tissue without a concomitant increase in CLA intake has several possible explanations. The increase may have resulted from a reduction in CLA oxidation. Another possibility includes a lower digestibility of CLA, when supplemental fat is excluded, because the added CLA was fed as a free fatty acid (FFA) and FFA intake has been shown to affect digestibility in a negative manner (35
). Alternatively, de novo fatty acid synthesis may have been reduced by supplemental fat feeding, allowing a greater deposition of dietary fatty acids (including CLA) compared with pigs consuming 1 g/100 g CLA without supplemental fat. Deposition of CLA in the latter group of pigs may be diluted by fatty acids synthesized de novo and stored in adipose tissue. Additional research is required to explain this important finding.
A linear relationship between the PUFA content of the feed and the PUFA content of both the backfat and intramuscular fat tissue appears to exist, although there are some differences in the incorporation rates of the two depots (4
,21
). In line with this finding, both supplemental fat and CLA addition to the diets resulted in changes to the fatty acid composition of belly fat and longissimus dorsi intramuscular fat. CLA supplementation increased 14:0, 16:0, 18:0 and 18:1 trans-9 and decreased 18:1 cis-9 and 20:1 cis-11 in belly fat. In addition, the amounts of MUFA were reduced and the MUFA/PUFA ratio was increased by CLA feeding. Most other studies that have determined fatty acid composition after supplementing 1–2 g/100 g CLA have measured similar changes (9
). In addition to measuring an increase in stearic acid, we noted a corresponding decrease in oleic acid in fat samples from CLA-supplemented pigs. Ramsay et al. (34
) showed similar results with apparent increases in the relative percentages of stearic acid and a reduction in oleic acid. They and others noted that 1–2 g/100 g dietary CLA may inhibit 9 desaturase activity in both skeletal muscle and adipose tissue of several species (15
, 36
). The 9 desaturase enzyme catalyzes the synthesis of oleic acid (18:1 cis-9) from stearic acid (18:0); therefore, it is an important factor in determination of the levels of these fatty acids in porcine adipose tissue. This enzyme also has activity for synthesis of 14:1 cis-9 and 16:1 cis-9 from 14:0 and 16:0, respectively. CLA has been also been shown to reduce fat deposition and increase carnitine palmitoyl transferase enzyme activity in mice (13
,37
). Increases in the cis-9, trans-11 and the trans-10, cis-12 CLA isomers from belly fat of pigs in this study have been seen in other studies (8
,9
). When CLA concentration was increased linearly in diets from 0 to 1 g/100 g, a linear increase in the CLA content in both subcutaneous pork adipose tissue and lean tissue was found (8
). Because CLA has the potential to alter gene expression of key lipogenic enzymes (13
–15
) and supplemental fat can be directly deposited in swine adipose tissue, their combination may result in additive effects on pork fat quality. Indeed, we noted that supplementation of 1 g/100 g CLA and 4 g/100 g tallow resulted in an additive reduction of belly fat iodine value (IV) (Figure 2A)
. Before this study, we and others have noted increased saturated fatty acid composition due to tallow supplementation (2
,4
,38
). Pigs fed diets containing extruded full-fat soybeans or 4 g/100 g tallow for 6 wk exhibited a 39.9% reduction in LA of bacon samples from the pigs consuming the 4 g/100 g tallow diet compared with those consuming the 20 g/100 g full-fat soybean diet (2
).
In our experiment, the 12.5% increase in LA from belly fat of pigs consuming 4 g/100 g yellow grease is not surprising. Boyd (39
) compared the relationships between the fatty acid profile of the diet and the resulting profile and IV of backfat. He determined that there was a linear relationship between dietary LA content and the IV of backfat. These same relationships can be seen in this study. The yellow grease had a greater 18:2 cis-9, cis-12 content than the tallow, and tallow supplementation reduced the 18:2 cis-9, cis-12 content in both the belly fat and the longissimus muscle intramuscular fat. Other data collected in our laboratory have shown a linear decrease in 18:2 cis-9, cis-12 content and IV of carcass fat as dietary tallow level increased (4
).
Both saturated fat and CLA have been shown to increase belly firmness (4
,8
, 9
) and have the potential to affect pork quality. The reduced IV and increased belly weights of pigs fed 1 g/100 g CLA in our study support an improvement in belly firmness. Eggert et al. (9
) measured belly firmness on a scale from 1 (very soft) to 3 (very firm) in pigs fed 1 g/100 g CLA from 90 to 115 kg. Firmness was increased by 0.5 units in the CLA-fed pigs compared with those consuming diets with 1 g/100 g sunflower oil and by 0.8 units compared with those fed 1 g/100 g sunflower oil and restricted to the intake of CLA-fed pigs. Of the pork quality work completed, the main focus has been on the effect of CLA on longissimus thoracis quality and palatability (10
,40
). Several pork quality attributes, including marbling and intramuscular fat content, may be altered by CLA and dietary fat supplementation (40
). The marbling score of the longissimus chops was increased 18.8% in pigs fed CLA (Table 3)
. Others have shown similar results with increases of 11.3% with CLA supplementation (41
). Related to the increase in marbling score was a 2.77-g increase in intramuscular fat, partially resulting in an 18.0 g/kg wet loin increase in lean (42
). Joo et al. (43
) noted a reduction in purge loss of samples kept in cold storage for 7 d from pigs fed a 5 g/100 g CLA diet. This was related to an increase in intramuscular fat content and may be attributed to an increase in the resistance of membrane lipids to oxidation (43
).
Several researchers have observed a reduction in backfat depth with CLA feeding (8
,10
,11
,12
). However, backfat depth in our study was not altered by CLA or supplemental fat and averaged only 14 mm at slaughter. Decreases in mRNA expression of fatty acid synthase, steroyl-coA desaturase and acetyl co-A carboxylase have been reported in mammary tissue of cows fed the trans-10, cis-12 CLA isomer, indicating that lipogenesis and fat deposition may be reduced by CLA (14
). Backfat thickness in carcasses from pigs fed 4.8 or 9.5 g/kg CLA was reduced 24% compared with pigs receiving no CLA. However, backfat depths in these experiments were much greater (16–28 mm) than in our study. In addition, no effects of CLA were detected on the percentage of fat-free lean. Perhaps the pigs in our study were approaching a minimum backfat depth required for normal tissue structure and function and this may have resulted in increased resistance to further changes. Ramsay et al. (34
) reported similar results in growing pigs consuming CLA and treated with porcine somatotropin. They did not detect an effect of CLA on body composition or on backfat depth. Along with our data, this would indicate that the greatest effect of CLA supplementation on body composition or total lipid content would be in pigs that are in the final stages of finishing or accumulating fat at a higher rate (34
). Wood (44
) posed two possibly relevant explanations, i.e., there may be a difference in the mechanism of fat deposition in genetically fat vs. genetically lean pigs or the proportion of de novo fatty acid synthesis (usually more saturated) is reduced in lean pigs along with an overall lower fat deposition at the same level of feed intake.
In summary, CLA enrichment of pork products may be enhanced when CLA feeding is combined with supplemental dietary fat in lean-genotype swine. In addition, data herein showed that, individually, tallow and CLA increased the saturation of belly fat and when supplemented together, reduced belly fat IV from 70 to 62 within 6 wk. Negative effects of tallow or CLA supplementation on growth, feed intake, feed efficiency or carcass quality were not detected. Fatty acid composition of belly fat was altered by both tallow and CLA addition. However, supplementation of yellow grease increased the LA in belly fat samples and could increase the opportunity for lipid oxidation in pork products. Further research is warranted to investigate the metabolic basis for the extra CLA enrichment associated with dietary fat supplementation. Similarly, depot-specific effects (e.g., belly vs. backfat vs. intramuscular fat) merit further examination so that value-added enrichment of pork products may be optimized.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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2 Supported in part by the Fats and Proteins Research Foundation, Bloomington, IL, the North Carolina Pork Producers Council and the North Carolina Agricultural Research Service of North Carolina State University, Raleigh, NC.
4 Abbreviations used: CLA, conjugated linoleic acid; FFA, free fatty acid; IV, iodine value; LA, linoleic acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid; U/S, unsaturated to saturated fat ratio; wt, weight.
Manuscript received 21 February 2002. Initial review completed 25 March 2002. Revision accepted 19 July 2002.
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LITERATURE CITED |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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1. American Heart Association Conference Proceedings (2001) Summary of the scientific conference on dietary fatty acids and cardiovascular health: conference summary from the nutrition committee of the American Heart Association. Circulation 103:1034-1039.
2. Viljoen, J. & Ras, M. N. (1991) The effect of different dietary fat sources, withdrawal times, and castration on the fatty acid composition of backfat in baconer pigs. South Afr. J. Anim. Sci. 21:76-79.
3. Averette Gatlin, L. A. (2001) Improving Pork Quality in Lean Genotype Swine: Effects of Chemical Hydrogenation of Dietary Fat and Supplementation of Conjugated Linoleic Acid. Doctoral thesis 2001 North Carolina State University Raleigh, NC. .
4. Averette Gatlin, L. A., See, M. T., Hansen, J. A., Sutton, D. & Odle, J. (2002) The effects of dietary fat sources, levels, and feeding intervals on pork fatty acid composition. J. Anim. Sci. 80:1606-1615.
5. Ip, C., Chin, S. F., Scimeca, J. A. & Pariza, M. W. (1991) Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res. 51:6118-6124.
6. Rose, D. P., Rayburn, J., Hatala, M. A. & Connolly, J. M. (1994) Effects of dietary fish oil on fatty acids and eicosanoids in metastasizing human breast cancer cells. Nutr. Cancer 22:131-141.
7. Lee, K. N., Kritchevsky, D. & Pariza, M. W. (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25.[Medline]
8. Thiel-Cooper, R. L., Parrish, F. C., Jr, Sparks, J. C., Wiegand, B. R. & Ewan, R. C. (2001) Conjugated linoleic acid changes swine performance and carcass composition. J. Anim. Sci. 79:1821-1828.
9. Eggert, J. M., Belury, M. A., Kempa-Steczko, A., Mills, S. E. & Schinckel, A. P. (2001) Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs. J. Anim. Sci. 79:2866-2872.
10. Wiegand, B. R., Parrish, F. C., Jr, Swan, J. E., Larsen, S. T. & Baas, T. J. (2001) Conjugated linoleic acid improves feed efficiency, decreases subcutaneous fat, and improves certain aspects of meat quality in Stress-Genotype pigs. J. Anim. Sci. 79:2187-2195.
11. Wiegand, B. R., Sparks, J. C., Parrish, F. C. & Zimmerman, D. R. (2002) Duration of feeding conjugated linoleic acid influences growth performance, carcass traits, and meat quality of finishing barrows. J. Anim. Sci. 80:637-643.
12. Ostrowska, E., Muralitharan, M., Cross, R. F., Bauman, D. E. & Dunshea, F. R. (1999) Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129:2037-2042.
13. Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E. & Pariza, M. W. (1999) Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids 34:243-248.[Medline]
14. Bauman, D. (2001) Update on conjugated linoleic acids (CLA). Cornell Nutrition Conference Proceedings. Oct. 16–18 2001 Ithaca, NY. .
15. Lee, K. N., Pariza, M. W. & Ntambi, J. M. (1998) Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem. Biophys. Res. Commun. 248:817-821.[Medline]
16. National Research Council (1998) Nutrient Requirements of Swine 10th ed. 1998 National Academy Press Washington, DC. .
17. National Pork Board (2000) Berg, E. eds. Pork Composition and Quality Assessment Procedures 2000:1-38 National Pork Producers Council Des Moines, IA. .
18. Kauffman, R. G., Eilelenboom, G., van der Wal, P. G. & Merkus, G. (1986) The use of filter paper to estimate drip loss of porcine musculature. Meat Sci. 18:191-200.
19. Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
20. AOCS (1998) Recommended practice Cd 1c-85. Official Methods and Recommended Practices of the AOCS 5th ed. 1998 AOCS Champaign, IL. .
21. Warnants, N., VanOeckel, M. J. & De Paepe, M. (2001) Fat in pork: image, dietary modification and pork quality. Pig News Info. 22:107N-113N.
22. Roberfroid, M. B. (1999) Concepts in functional foods: the case of inulin and oligofructose. J. Nutr. 129:1389S-1401S.
23. NPPC (1999) 98/99 Pork Facts 1999 Publication of the National Pork Producers Council Des Moines, IA. http://www.nppc.org/PorkFacts/pfindex.html (accessed 28 January 2002).
24. Morgan, J. B., Smith, G. C., Cannon, J., McKeith, F. & Heavner, J. (1994) Pork distribution channel audit report. Meeker, D. Sonka, S. eds. Pork Chain Quality Audit—Progress Report 1994 NCCP Des Moines, IA. .
25. Scott, R. A., Cornelius, S. G. & Mersmann, H. J. (1983) Fatty acid composition of adipose tissue from lean and obese swine. J. Anim. Sci. 53:977.
26. Pettigrew, J. A. & Esnaola, M. A. (2000) Swine nutrition and pork quality. Fact Sheet 04458–2 2000 National Pork Board Des Moines, IA. .
27. National Pork Board (2000) 01/02 Pork Facts 2000:10www.porkboard.org/prodissues/porkfacts.asp (accessed 28 January 2002).
28. Wood, J. D. & Enser, M. (1989) Fat quality in pigs with special emphasis on genetics. 40th Annual Meeting of the Study Commissions EAAP, Commissions on Animal Genetics and Pig Production. August 17–31, 1989 1989 Dublin, Ireland. .
29. Brasitus, T. A., Davidson, N. O. & Schachter, D. (1985) Variations in dietary triacylglycerol saturation alter the lipid composition and fluidity of rat intestinal plasma membranes. Biochem. Biophys. Acta 812:460-472.[Medline]
30. Kouba, M. & Mourot, J. (1999) Effect of a high linoleic acid diet on lipogenic enzyme activities and on the composition of the lipid fraction of fat and lean tissues in the pig. Meat Sci. 52:39-45.
31. Kelly, M. L. & Bauman, D. E. (1996) Conjugated linoleic acid: potent anticarcinogen found in milk fat. Proc. Cornell Nutr. Conf. 1996 Cornell University Ithaca, NY. .
32. Whigham, L. D., Cook, M. E. & Atkinson, R. L. (2000) Conjugated linoleic acid: implications for human health. Pharm. Res. 42:503-510.
33. Benito, P., Nelson, G. J., Kelley, D. S., Bartolini, G., Schmidt, P. C. & Simon, V. (2001) The effect of conjugated linoleic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids 36:229-236.[Medline]
34. Ramsey, T. G., Evock-Clover, C. M., Steele, N. C. & Azain, M. J. (2001) Dietary conjugated linoleic acid alters composition of pig skeletal muscle and fat. J. Anim. Sci. 79:2152-2161.
35. Powles, J., Wiseman, J., Cole, D.J.A. & Hardy, B. (1993) Effect of chemical structure of fats upon their apparent digestible energy value when given to growing/finishing pigs. Anim. Prod. 57:137-146.
36. Kouba, M. & Mourot, J. (1998) Effect of a high linoleic acid diet on -9 desaturase activity, lipogenesis and lipid composition of pig subcutaneous adipose tissue. Reprod. Nutr. Dev. 38:31-37.
37. Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E. & Pariza, M. W. (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858.[Medline]
38. Leszczynski, D. E., Pikul, J., Easter, R. A., McKeith, F. K., McLaren, D. G., Novakofski, J., Bechtel, P. J. & Jewell, D. E. (1992) Characterization of lipid in loin and bacon from finishing pigs fed full-fat soybeans or tallow. J. Anim. Sci. 70:2175-2181.[Abstract]
39. Boyd, R. D. (1997) Relationship between dietary fatty acid profile and body fat composition in growing pigs. PIC USA T & D Technical Memo 153 1997 Pig Improvement Company USA, Franklin, KY. .
40. Dugan, M.E.R., Aalhus, J. L., Jeremiah, L. E., Kramer, J.K.G. & Schaefer, A. L. (1999) The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J. Anim. Sci. 79:45-51.
41. Dugan, M.E.R., Aalhus, J. L., Schaefer, A. L. & Kramer, J.K.G. (1997) The effect of fat to lean repartitioning and feed conversion in pigs. Can. J. Anim. Sci. 77:723-725.
42. Dugan, M.E.R. & Aalhus, J. L. (1999) Feeding CLA to pigs: effects on feed conversion, carcass composition, meat quality and palatability. Yurawecz, M. P. eds. Advances in Conjugated Linoleic Acid Research 1999:354-368 Iowa State University Press Ames, IA. .
43. Joo, S. T., Lee, J. I., Ha, Y. L. & Park, G. B. (2002) Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color, and water-holding capacity of pork loin. J. Anim. Sci. 80:108-112.
44. Wood, J. D. (1973) The fatty acid composition of backfat from Pietrain and Large White pigs. Anim. Prod. 17:281-285.
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