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Journal of Nutrition Vol. 99 No. 4 December 1969, pp. 465-473
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Metabolism of Tryptophan in Isolated Perfused Rat Liver1, 2,

C. Y. Ng3, Y. Hagino4, P. B. Swan and L. M. Henderson

Department of Biochemistry, College of Biological Sciences and Division of Nutrition, College of Agriculture, Forestry and Home Economics, University of Minnesota, St. Paul, Minnesota 55101

The fate of tryptophan in the isolated perfused rat liver was investigated. It was shown that the liver removed the L-isomer more rapidly from the perfusate than the D-isomer. The 10 g liver was capable of a removal rate of 35 mg/hour when 42 mg of DL-tryptophan was given initially, followed by infusion at the rate of 42 mg/hour. Carbon-14 from DL-tryptophan-2-14C appeared in CO2 much more rapidly and to a greater extent within 3 hours than did that from DL-tryptophan-7a-14C. In contrast to whole-animal studies, it was shown that overloading doses of acetoacetate were labeled by DL-tryptophan-5- or 7a-14C. The labeling pattern in acetoacetate was similar to that observed when 14C-fatty acids are the substrate. Acetoacetate overloading suppressed the production of radioactive carbon dioxide from DL-tryptophan-7a-14C.

1 Supported by Grant no. GM 11710 from the National Institutes of Health and by funds provided by the Minnesota Agricultural Experiment Station. Scientific Journal Series No. 6991, Agricultural Experiment Station, University of Minnesota, St. Paul, Minnesota.

2 Presented in part at the Seventh International Congress of Biochemistry, Tokyo, Japan, August, 1967. Metabolism of tryptophan by perfused rat liver, 1968. Medical Journal of Osaka University, 19: 25 (abstract).

3 Deceased.

4 Present address: Nagoya University, School of Medicine, Nagoya, Japan.

Manuscript received 17 July 1969.




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