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Effect of Iron on Growth, Cytochromes, Glycogen and Fatty Acids of Tetrahymena pyriformis^1,2,

Veterans Administration Hospital, and Departments of Nutritional Sciences and Medicine, University of Wisconsin, Madison, Wisconsin

Although the iron concentration in 2% proteose-peptone was found to be about the same as in chemically defined media, the addition of inorganic iron salts produced a dramatic acceleration of growth and marked alterations in the metabolism of the ciliated protozoan, Tetrahymena pyriformis. In well aerated cultures, iron supplementation resulted in marked increases in pyridine hemochromogen concentration, conversion of heme to cytochrome, glycogen concentration, degree of unsaturation of fatty acids and cell population.

² This manuscript is approved for publication by the Director of the Research Division of the College of Agricultural and Life Sciences, University of Wisconsin.

Manuscript received 30 June 1969.