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Effect of Urease Immunity on Urease and Antiurease Activities in Ruminants^1,2,

Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma

Jackbean urease was extracted and recrystallized three times. Subcutaneous injection of urease in sheep produced urease antibodies in blood and certain secretions. Concurrently urease activity in rumen fluid was reduced. Antiserum gave a positive precipitin ring reaction with ruminal urease. A surgical technique for the cannulation of the parotid duct of sheep is described.

² Partial financial support provided by General Medical Research Grant no. FR-05567.

³ Department of Animal Sciences and Industry.

⁴ Present address: Biology Department, Phillips University, University Station, Enid, Oklahoma 73701.

⁵ Department of Veterinary Medicine and Surgery.

Manuscript received 3 March 1969.