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Effect of Thiamine Deficiency in Rats on Adipose Tissue Lactate Dehydrogenase Isozyme Distribution¹

Biochemistry Department, The Ohio State University, Columbus, Ohio

Lactate dehydrogenase (LDH) (EC. 1.1.1.27) was present in five isozymic forms in rat epididymal adipose tissue from rats fed a complete diet; LDH-I (most anodal electrophoretically) was present in low concentration. Thiamine deficiency resulted in a reduction of specific activity (units per milligram soluble protein) of total LDH, but a marked increase in LDH-I and LDH-II. Feeding thiamine-deficient rats a complete diet restored the LDH-isozyme distribution pattern to control characteristics. The shift in LDH-isozyme distribution pattern with thiamine deficiency was suggestive of an increase in the activity of the gene responsible for the synthesis of the H subunit.

Manuscript received 3 February 1969.