Journal of Nutrition OpenSOurce Diets- www.ResearchDiets.com

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 96 No. 4 December 1968, pp. 433-444
Copyright © 1968 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuoki, Z.
Right arrow Articles by Miyakawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuoki, Z.
Right arrow Articles by Miyakawa, M.

Metabolism of Thiamine and Thiamine Tetrahydrofurfuryl Disulfide to 4-Methylthiazole-5-Acetic Acid in Conventional and Germfree Rats under Various Dosing Conditions1

Ziro Suzuoki, Fumio Tominaga, Takao Matsuo, Yukiko Sumi2 and Masasumi Miyakawa2

Biological Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Osaka, and Laboratory of the Germfree Life Research, School of Medicine, Nagoya University, Nagoya, Japan

The urinary excretion of thiamine and 4-methylthiazole-5-acetic acid was studied in both conventional and germfree rats under various dosing conditions of thiazole-2-14C-labeled thiamine or thiamine tetrahydrofurfuryl disulfide. The urine was fractionated by Amberlite CG-50 chromatography and the thiazole acid was determined by the isotope dilution method. Thiamine, in an oral dose larger than 1.5 mg/rat, was predominantly excreted in the urine after conversion to the thiazole acid, whereas most of the injected thiamine was eliminated unchanged. The thiamine moiety of thiamine tetrahydrofurfuryl disulfide was also converted to the thiazole acid upon oral ingestion but less was excreted as the thiazole acid and more as the unchanged thiamine, comparing with the administration of thiamine. Time course study on the urinary metabolite pattern showed that most of the thiazole acid was excreted during the first 3 hours after oral ingestion of both vitamins. The thiazole acid was also identified as a metabolite of thiamine in germfree rats and there was no essential difference in the urinary metabolite pattern between conventional rats and germfree ones. This finding indicates that intestinal microflora are not primarily responsible for the conversion of thiamine to the thiazole acid in the rat.

1 A part of this paper was presented at the 179th Meeting of the Japan Vitamin B Research Committee (December 9, 1967, in Nagoya, Japan).

2 Laboratory of the Germfree Life Research, Nagoya University.

Manuscript received 1 April 1968.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]