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Effect of Ascorbic Acid on Fluoride Uptake in the Polymorphonuclear Leukocyte of the Guinea Pig¹

Faculty of Food Sciences and Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, and Department of Chemistry, University of Guelph, Guelph, Canada

Radiotracer fluorine-18 was produced, isolated, and used as a physiological tracer in an investigation of the effect of ascorbic acid on the uptake of fluoride by exudate leukocytes of the guinea pig. It was possible to measure fluoride uptake to within ± 0.0005 µg/10⁷ cells by this method. When the leukocytes were incubated at concentrations of 2.4 ppm and 1.2 ppm fluoride, the cells containing high levels of ascorbic acid (more than 0.9 µg ascorbic acid/10⁷ cells) took up significantly more fluoride than those containing low levels (less than 0.2 µg ascorbic acid/10⁷ cells). Addition of ascorbic acid to the medium did not cause an increase in the uptake of fluoride. With incubation in 2.4 ppm fluoride both types of cells took up approximately twice as much fluoride as they did at 1.2 ppm.

² Faculty of Food Sciences, University of Toronto, Canada.

³ Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada, to whom reprint requests may be addressed.

⁴ Department of Chemistry, University of Guelph, Canada, to whom reprint requests may be addressed.

Manuscript received 19 January 1968.