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Journal of Nutrition Vol. 96 No. 1 September 1968, pp. 10-14
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Choline Biosynthesis in Germfree Rats1

Eva Kwong, Grace Fiala, Richard H. Barnes, Dorinne Kan and Stanley M. Levenson

Graduate School of Nutrition, Cornell University, Ithaca, New York, and Department of Surgery, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York

Liver choline biosynthesis was studied in germfree rats receiving a choline-deficient diet. Weanling conventional open-animal-room and ex-germfree conventionalized rats fed the choline-deficient diet for 2 weeks had elevated liver fat and decreased specific activities of liver choline following injection of 2 µCi14CH3-labeled methionine into the portal vein. On the other hand, in germfree rats fed the cholinedeficient diet there was no significant increase in liver fat nor was there a decrease in the rate of liver choline biosynthesis as measured by labeled methyl transfer. In these respects germfree rats fed a choline-deficient diet appeared to be the same as control rats fed a diet with adequate choline. These findings are consistent with a previous observation of a lessened nephropathy in germfree rats fed a choline-deficient diet, and also are similar to results obtained with conventional rats in which coprophagy had been prevented.

1 At Cornell University this research was supported in part by funds provided through the State University of New York and a research grant from the National Science Foundation. At Albert Einstein College of Medicine, Yeshiva University, this research was supported in part by National Institutes of Health Grants no. 5P01 AM 05664 AMP and 5-K5-GM-14,20805 (Career Research Award to Dr. Stanley M. Levenson).

Manuscript received 29 February 1968.




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