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Response of Lipogenesis to Repletion in the Pyridoxine-deficient Rat^1,2,

Department of Nutrition, University of Toronto, Toronto, Canada

Pyridoxine has been implicated in fat metabolism. To elucidate its role the response of lipogenetic activities in the liver and epididymal pad of deficient rats to pyridoxine administration was measured. Rats were fed a pyridoxine-deficient diet until they ceased to gain weight (40 days); they were then repleted by the subcutaneous injection of 300 µg pyridoxine·HCl/rat every day for 5 days. During repletion, diets varying in fat content (0 to 25%) were fed. The administration of the vitamin resulted in increases in the liver and epididymal glucose 6-phosphate dehydrogenase which were small in the pair-fed group and substantial in the ad libitum-fed controls. The enzyme activity was highest (five fold) in rats receiving the fat-free diet. The substitution of starch for sucrose resulted in lower levels of dehydrogenase acitvity. In animals fed ad libitum and repleted with the same basal diet, the liver lipids, the hepatic glucose 6-phosphate dehydrogenase activity and the extent of acetate-¹⁴C incorporation into liver lipids were high on day 2 of repletion and returned to normal levels by day 11 with a concomitant increase in carcass fat, approaching the levels in controls maintained with a pyridoxine-sufficient diet through-out the experiment. The data demonstrate that the role of pyridoxine in lipogenesis may be mediated through the increase in food intake with the repletion of the vitamin.

² Supported in part by grants from the Medical Research Council and the National Research Council of Canada and the Williams-Waterman Fund, New York.

³ Permanent address: Biochemistry Division, Central Food Technological Research Institute, Mysore 2, India.

Manuscript received 20 December 1967.