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Journal of Nutrition Vol. 94 No. 2 February 1968, pp. 227-232
Copyright © 1968 by American Society for Nutrition
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Destruction of Rachitogenic Activity of Isolated Soybean Protein by Autoclaving as Demonstrated with Turkey Poults1, 2,

O. J. Thompson, C. W. Carlson, I. S. Palmer and O. E. Olson

South Dakota State University, Brookings, South Dakota

Investigations were conducted to determine the effect of increasing vitamin D3 levels and the effect of autoclaving on the rachitogenic activity of isolated soybean protein (C-1 protein—Skidmore). One-day-old poults were fed purified diets of the glucose-C-1 protein type. The diets contained calcium, phosphorus and vitamin D3 at levels equal to or greater than National Research Council recommendations. These results demonstrate that autoclaving destroys rachitogenic activity exhibited by C-1 protein. Autoclaving C-1 protein increased tibia ash values, and concomitantly serum alkaline phosphatase activity was decreased. However, autoclaving the C-1 protein for 60 minutes was most effective in reducing the rachitogenic effects of C-1 protein. In 2 out of 3 experiments, use of autoclaved C-1 protein in the diet also increased weight gains and serum calcium levels. In general, increasing dietary levels of vitamin D3 lowered serum alkaline phosphatase activity. The effects of increasing vitamin D3 levels on weight gains, bone ash and serum calcium were variable between experiments. Neither autoclaved C-1 protein nor increasing vitamin D3 levels had any effect on serum inorganic phosphate.


1 This investigation was supported in part by the Public Health Service Research Grant AM-08479-02 from the National Institute of Arthritis and Metabolic Diseases.

2 Approved for publication by the Director of the South Dakota Agricultural Experiment Station as Paper no. 778 of the Journal series.

Manuscript received 10 July 1967.





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