QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lopez, P. L. Articles by Pfander, W. H. Search for Related Content PubMed PubMed Citation Articles by Lopez, P. L. Articles by Pfander, W. H.

In vitro Uptake of Selenium-75 by Red Blood Cells from the Immature Ovine during Varying Selenium Intakes^{1, 2,}

Department of Animal Husbandry, University of Missouri, Columbia, Missouri

Six crossbred wether lambs were used for preliminary study of the effect of varying Se intake upon uptake of ⁷⁵Se in vitro by ovine red blood cells. In a second experiment, 16 crossbred wether lambs were equally allotted among 4 dietary Se levels of 0.014, 0.264, 0.514 and 5.014 ppm. The feeding trial lasted 104 and 107 days for experiments 1 and 2, respectively. Lambs receiving 0.264 and 0.514 ppm Se gained faster, consumed more feed and had a higher feed efficiency than lambs in other dietary groups. Lambs on 0.014 ppm Se performed better than with the 5.014 ppm level. Adequate vitamin E may have prevented deficiency syndromes in lambs fed the low level of Se. Two lambs on the 5.014 ppm level exhibited symptoms of chronic selenosis. The uptake of ⁷⁵Se was significantly correlated with incubation time in the presence of an O₂-CO₂ mixture. The initial influx and rate of in vitro uptake of ⁷⁵Se by ovine blood cells decreased with increasing Se level in the diet. The in vitro uptake of ⁷⁵Se by red blood cells proved to be a promising technique to assess the nutritional Se status in the immature ovine.

² Part of doctoral dissertation of the senior author, Department of Animal Husbandry, University of Missouri, Columbia, Missouri 65201.

Manuscript received 13 May 1967.