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Ohio Agricultural Research and Development Center, Wooster, Ohio
The repression of liver malic enzyme induced by adding 0.025% iodinated casein (1% thyroxine (T4) equivalent) to the basal diet was used to measure the antithyrotoxic factor (ATF) content of several roughages (flaked soybean hulls, alfalfa hay, corn silage and alfalfa-grass silages) and dried cow feces fed at the level of 15% in the diet of rats. Relative ATF units (100 units equivalent to the effect of adding 10% hemoglobin standard to the thyrotoxic diet) ranged from 77.6 to 116.3 in the roughages, soybean hulls being lowest and alfalfa-grass silage highest. The ATF content of the dried feces from cows fed alfalfa-grass silages was higher than that of the silages, ranging from 125.3 to 134.9 units.
2 Approved for publication as Journal Article no. 76-67 by the Associate Director, Ohio Agricultural Research and Development Center, Wooster.
3 Present address: Departments of Pediatrics and Physiological Chemistry, The Ohio State University, College of Medicine, Columbus.
Manuscript received 7 August 1967.