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Journal of Nutrition Vol. 94 No. 2 February 1968, pp. 125-128
Copyright © 1968 by American Society for Nutrition
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Antithyrotoxic Factor Content of Various Roughages and Cow Feces Based on Repression of Induced Liver Malic Enzyme in Thyrotoxic Rats1, 2,

Somasundaram Addanki3, J. W. Hibbs and H. R. Conrad

Ohio Agricultural Research and Development Center, Wooster, Ohio

The repression of liver malic enzyme induced by adding 0.025% iodinated casein (1% thyroxine (T4) equivalent) to the basal diet was used to measure the antithyrotoxic factor (ATF) content of several roughages (flaked soybean hulls, alfalfa hay, corn silage and alfalfa-grass silages) and dried cow feces fed at the level of 15% in the diet of rats. Relative ATF units (100 units equivalent to the effect of adding 10% hemoglobin standard to the thyrotoxic diet) ranged from 77.6 to 116.3 in the roughages, soybean hulls being lowest and alfalfa-grass silage highest. The ATF content of the dried feces from cows fed alfalfa-grass silages was higher than that of the silages, ranging from 125.3 to 134.9 units.


1 From a dissertation submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree, The Ohio State University, Columbus, 1964.

2 Approved for publication as Journal Article no. 76-67 by the Associate Director, Ohio Agricultural Research and Development Center, Wooster.

3 Present address: Departments of Pediatrics and Physiological Chemistry, The Ohio State University, College of Medicine, Columbus.

Manuscript received 7 August 1967.





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