QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Brin, M. Articles by Thiele, V. F. Search for Related Content PubMed PubMed Citation Articles by Brin, M. Articles by Thiele, V. F.

Relationships between Vitamin B₆-vitamer Content and the Activities of Two Transaminase Enzymes in Rat Tissues at Varying Intake Levels of Vitamin B₆^{1 ,2}

Upstate Medical Center, State University of New York, Syracuse, New York and the Department of Foods and Nutrition, Syracuse University, Syracuse, New York

To study relationships between the vitamin B₆-vitamer content of rat tissues to the activities of 2 transaminase enzymes in those tissues, and the relative biological activity of 3 vitamin B₆-vitamers in the rat, we arranged to study, in depth 7 groups of rats that were fed diets containing (µg/g diet) 0, 0.5, 1.0, 2.0, and 10.0 of pyridoxol·HCl and 1.0 of pyridoxal·HCl or pyridoxamine·2HCl. Although a dietary level of 2.0 µg/g diet of pyridoxol was adequate to support maximal growth, higher tissue levels of total vitamin B₆ and of the individual vitamers were reached at the 10.0 µg/g diet level, in liver, kidney, brain, muscle, and heart. Of the 3 vitamin B₆ vitamers, pyridoxamine was depleted to the greatest extent, as percentage, at lower levels of dietary pyridoxol. In studies on glutamic-pyruvic (GPT) and glutamic-oxalacetic transaminase (GOT) activities in the above tissues plus plasma and red blood cells, enzyme activity increased with increased dietary pyridoxol and with increased tissue vitamin B₆ content. GPT was depleted to a greater extent than, and GOT to a lesser extent than, the percentage depletion for tissue vitamin B₆ at lower levels of pyridoxol intake. It appeared from studies on growth, tissue content of vitamin B₆ and vitamers, and tissue transaminase activity, that at the level of 1.0 µg/g diet, pyridoxal was essentially equivalent to pyridoxol for vitamin B₆ activity; the study on pyridoxamine required re-evaluation. A simplified medium was used for the microbiological portion of the assay of the vitamin B₆ vitamers.

² Presented in part at the meetings of the Federation of American Societies for Experimental Biology, Chicago, 1967.

Manuscript received 10 July 1967.