Journal of Nutrition

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Journal of Nutrition Vol. 93 No. 2 October 1967, pp. 142-152
Copyright © 1967 by American Society for Nutrition
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Effect of Dietary Fat and Cholesterol on the in vitro Incorporation of Acetate-14C into Hen Liver and Ovarian Lipids1

Joseph F. Weiss, Edward C. Naber and Ralph M. Johnson

Institute of Nutrition and the Departments of Poultry Science and Physiological Chemistry, The Ohio State University, Columbus, Ohio and The Ohio Agricultural Research and Development Center, Wooster, Ohio

A study was made of the incorporation of acetate-1-14C into the lipid fractions of liver slices and ovarian tissues from hens fed a basal diet and diets containing either safflower oil (30%), animal fat (30%), cholesterol (1%), or cholesterol (1%) and safflower oil (29%). When safflower oil was fed, acetate, incorporation into total liver lipids decreased. The distribution of 14C (ratio of 14C in a particular fraction to the 14C in total lipids) increased in the cholesterol and phospholipid fractions, decreased in triglycerides, and remained the same in diglycerides and cholesterol esters. When animal fat was fed, the distribution of 14C increased in the cholesterol fraction and decreased in triglycerides, but total lipid synthesis was depressed further than it was when safflower oil was fed. When cholesterol was fed, acetate incorporation into liver cholesterol decreased, while incorporation into triglyceride increased slightly. When both safflower oil and cholesterol were included in the diet, synthetic control by cholesterol predominated. Regulation of the pathways of lipid biosynthesis from acetate was observed in ovarian tissue only when the diet containing both cholesterol and safflower oil was fed. The implications of these results on the synthesis of egg yolk lipids are discussed.

1 Part 3 of a series entitled "The Effect of Dietary Fat and Other Factors on Egg York Cholesterol." A preliminary report of this investigation has been presented (Weiss, J. F., E. C. Naber and R. M. Johnson 1965 The effect of dietary fat and cholesterol on the incorporation of acetate into hen ovarian and liver lipids in vitro. Federation Proc., 24: 686). This work was supported in part by grants from the National Institutes of Health, U. S. Public Health Service (Training grant 5 T1 ES-17), the Ohio Poultry Research Fund, and the Ohio Agricultural Research and Development Center.

Manuscript received 29 April 1967.




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