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Journal of Nutrition Vol. 92 No. 4 August 1967, pp. 479-490
Copyright © 1967 by American Society for Nutrition
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Separation of Soybean Whey into Fractions with Different Biological Activities for Chicks and Rats1

Walther Sambeth2, M. C. Nesheim and J. A. Serafin

Department of Poultry Science and Graduate School of Nutrition, Cornell University, Ithaca, New York

Using a batch fractionation procedure on DEAE cellulose, 4 fractions of soybean whey were prepared and studied for biological activity with chicks and rats. All fractions prepared had trypsin inhibitor activity but hemagglutinin activity and intraperitoneal toxicity for rats was concentrated in one fraction. The fractions differed in relative stimulation of the pancreas of chicks to enlarge and the gallbladder to contract. Differences were also noted among the fractions in their effects on chick growth, fat absorption and dietary metabolizable energy value. Many of the effects caused by the soybean fractions appeared to be related to several components rather than to one specific fraction.

1 This investigation was supported in part by a Public Health Service Research Grant no. AM-8202 from the National Institute of Arthritis and Metabolic Diseases. In addition, one of us (W. S.) was supported during part of this work by a grant from the Ministry of Agriculture of the German Federal Republic.

2 Present address: Farbwerke Hoechst AG., Veterinärmedizinisches Laboratorium Frankfurt (M)-Hoechst/West Germany.

Manuscript received 20 March 1967.




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