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The Hormel Institute, University of Minnesota, Austin, Minnesota
Studies were conducted with chicks to determine changes in polyunsaturated fatty acids in liver lipids when the chicks were fed increasing amounts of dietary linoleate, and from this data the dietary requirement of the chick for linoleate was estimated. Increasing dietary amounts of linoleic acid fed to chicks as supplements of corn oil in a diet low in essential fatty acids (EFA) resulted in increased amounts of linoleate and arachidonate (18:2
6 and 20:4
6) and decreased amounts of eicosatrienoate (20:3
9) in liver lipids, a characteristic of EFA deficiency. The ratio of trienoate to tetraenoate was plotted to estimate the requirement of the chick for linoleate, which was found to be 2.0% of dietary calories. The requirement estimated from the rate constant equation by computer methods was found to be the same. Equations were derived to allow an estimate of the dietary linoleate intake, based on fatty acid composition of the liver lipid.
2 This investigation was supported in part by The Hormel Foundation and by Public Health Service Research Grants nos. AM-07123, HE-07508 and AM-04524 from the National Institutes of Health.
Manuscript received 16 February 1966.