Journal of Nutrition OpenSOurce Diets- www.ResearchDiets.com

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 88 No. 3 March 1966, pp. 284-290
Copyright © 1966 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berry, R. K.
Right arrow Articles by Wright, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berry, R. K.
Right arrow Articles by Wright, P. L.

Influence of Dietary Calcium, Zinc, and Oil upon the in vitro Uptake of Zinc-65 by Porcine Blood Cells1

R. K. Berry, M. C. Bell and P. L. Wright2

Agricultural Research Laboratory of the University of Tennessee, Oak Ridge, Tennessee,3

Twenty-four weanling barrows averaging 19 kg were allotted to 6 lots and fed at variable levels of either calcium, zinc, or fat for 16 weeks to determine effects on 65Zn retention by porcine blood cells in vitro. Five milliliters of freshly drawn whole blood were obtained at 2-week intervals and dosed with 200 mµc 65Zn. After incubation under O2 atmosphere at 38° for a 2-hour period, the cells were separated from the plasma and their radioactive zinc content was determined. Average percentage cellular 65Zn uptake per treatment after packed-cell volumes were adjusted to 40 were: basal, 34; basal + 71 ppm Zn, 26; basal + 0.6% Ca, 55; basal + 71 ppm Zn + 0.6% Ca, 28; basal + 5% oil, 34; basal + 0.6% Ca + 5% oil, 51. Dietary calcium increased (P < 0.01), whereas dietary zinc decreased (P < 0.01) in vitro uptake 65Zn throughout the experiment. Added oil exerted no significant effect on 65Zn uptake in vitro. Dietary treatment had no detectable effect on the stable zinc content of these cells. The effect of treatment upon the in vitro uptake of 75Sc, 137Cs, 131I, 60Co, 54Mn, 59Fe, 115Cd, and 64Cu by swine blood cells was also investigated with variable results. Four incidences of parakeratosis were observed in the 8 pigs fed the high calcium-low zinc diets.

1 This manuscript is published with the permission of the Director of the University of Tennessee Agricultural Experiment Station, Knoxville, Tennessee.

2 Present address: Monsanto Company, Agricultural Division, 800 N. Lindbergh Boulevard, St. Louis, Missouri.

3 Operated by the Tennessee Agricultural Experiment Station for the U. S. Atomic Energy Commission under Contract no. AT-40-1-GEN-242.

Manuscript received 23 August 1965.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]