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Autoradiographic Patterns of Methionine-2-¹⁴C and Methionine-Methyl-¹⁴C in Tissues of the Adult Rat^1,2,

Departments of Home Economics, Biology, and Chemistry, The Agricultural and Technical College of North Carolina, Greensboro, North Carolina

Using Kodak Type A autoradiographic plates, autoradiograms were prepared of 20 to 25 tissues from adult male Wistar rats killed 4 and 24 hours after feeding methionine-2-¹⁴C and 24 hours after feeding methionine-methyl-¹⁴C. The autoradiographic method used permitted the viewing on the same slide of the tissue alone, the autoradiogram alone and the tissue superimposed on its autoradiogram. With the exception of bone, all tissues produced autoradiograms at both time intervals; however, the 4-hour autoradiograms were much less intense than those produced after 24 hours. Epithelial tissue and bone marrow produced very intense autoradiograms. Muscle and connective tissue exhibited little activity. The uniformity of labeling observed when methionine-2-¹⁴C and methionine-methyl-¹⁴C were fed is interpreted to reflect the broad distribution of methionine and its metabolites in tissue proteins and other macromolecules rather than the existence of selective priority sites for compounds formed from its methyl or -carbon moieties.

² Paper no. 5 in the series: Utilization of Methionine by the Adult Rat.

Manuscript received 1 March 1965.