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Effect of Protein Depletion and Repletion in the Rabbit on the Activity of S³⁵ from Methionine in Serum Proteins and Urine^1,2,

Department of Preventive Medicine, School of Medicine and Department of Nutrition, Western Reserve University, Cleveland, Ohio

Sulfur-35 from injected methionine was measured in serum protein and urine of adult rabbits after dietary protein depletion and after repletion. Five rabbits fed a 4% casein diet for 39 days developed a hypochromic microcytic anemia, lost weight and were in negative nitrogen balance; however serum protein concentrations and fractions and blood volumes showed no significant change. During the same period there were no significant differences in these parameters between 2 groups of rabbits fed either a 20% casein ration or standard chow. In the depleted animals serum protein S³⁵ activity 8 hours after injection was higher, specific activity of the albumin fraction increased over 18 days, the percentage of the total activity persisting in the -globulin fraction was greater and more S³⁵/mg of urinary nitrogen was excreted although the total urinary S³⁵ was less. When the depleted animals were fed the 20% casein ration for 34 days, differences in nitrogen balance, red cell indexes, body weight and the utilization of the isotope disappeared. These results indicate that the isotope detected alterations in serum proteins that were not apparent by changes in concentration or albumin and globulin fractions.

² This work was supported in part by Public Health Service Research Grant no. HD-00669.

³ This work was presented in part at the annual meeting of the Federation of American Societies for Experimental Biology at Chicago, Illinois, 1964.

⁴ Formerly Predoctoral Fellow, National Institutes of Health, Public Health Service.

Manuscript received 11 September 1964.