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Journal of Nutrition Vol. 85 No. 3 March 1965, pp. 260-264
Copyright © 1965 by American Society for Nutrition
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Effect on Nitrogen Retention of Men of Varying the Total Dietary Nitrogen with Essential Amino Acid Intake Kept Constant1

Constance V. Kies2, Lucy Shortridge and May S. Reynolds

Department of Foods and Nutrition, School of Home Economics, University of Wisconsin, Madison, Wisconsin

Nitrogen balances of 6 young men on 3 different levels of total nitrogen intake but with identical intakes of purified essential amino acids plus cystine were determined. The essential amino acids plus cystine were given in the same amounts as found in 20 g egg protein. To bring the total nitrogen to the desired level, a solution of diammonium citrate and glycine was used in isonitrogenous amounts. The results indicated that 4 out of 6 subjects showed decreases in nitrogen loss as the daily nitrogen intake was increased from 4.0 to 6.0 g. All 6 subjects showed improved nitrogen retention when the total dietary nitrogen was increased to 8.0 g.

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. This is a contribution to North Central Regional Cooperative Project NC-49, Factors affecting requirements of adult human subjects for protein and amino acids. Data in this paper are taken from the dissertation submitted by Constance V. Kies in partial fulfillment of the requirements for the Degree of Doctor of Philosophy.

2 Present address: Department of Food and Nutrition, School of Home Economics, College of Agriculture and Home Economics, University of Nebraska, Lincoln.

Manuscript received 21 August 1964.




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