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Amino Acid Requirements of Children: Nitrogen Balance at the Minimal Level of Essential Amino Acids

Department of Nutrition and Biochemistry, Institute of Public Health, Tokyo, Japan

Minimal requirements of 8 essential amino acids previously determined separately, were fed in combination, along with the nonessential amino acids, to five 12-year-old boys for 20 days. Amino acid mixtures furnished 12.25 g nitrogen in the first 10 days, and 10.23 g in the last 10 days. Nitrogen balance and urinary excretion of creatine, creatinine, riboflavin and N-MNA were determined. All children maintained body weight at an almost constant level and a positive nitrogen balance throughout the experiment. Urinary excretion of creatinine remained almost constant, but that of creatine tended to increase during the feeding of the amino acid mixture. No significant trend was observed in urinary excretion of riboflavin and N-MNA.

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