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Amino Acid Requirements of Children: Minimal Needs of Tryptophan, Arginine and Histidine Based on Nitrogen Balance Method

Department of Nutrition and Biochemistry, Institute of Public Health, Tokyo, Japan

Minimal requirements for tryptophan, arginine and histidine of eleven 10- to 12-year-old boys were estimated by using the nitrogen balance method. Urinary excretion of creatine, creatinine, riboflavin and N-MNA was also measured. All children maintained a constant body weight and positive nitrogen balance without any accompanying clinical symptoms throughout the periods of arginine and histidine deprivation. With respect to tryptophan, all children maintained a positive balance at a level of 0.12 g, but some of them could not maintain a positive balance at the level of 0.06 g. The results obtained in all of our experiments with 10 amino acids in children have been summarized.

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