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Journal of Nutrition Vol. 79 No. 1 January 1963, pp. 37-44
Copyright © 1963 by American Society for Nutrition
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Effect of Acute Amino Acid Deficiencies on Carcass Composition and Pancreatic Function in the Force-fed Rat

II. Deficiencies of Valine, Lysine, Tryptophan, Leucine and Isoleucine1

Richard L. Lyman and Sue Stewart Wilcox

Department of Nutritional Sciences, University of California, Berkeley

Groups of rats were force-fed diets free of either valine, lysine, tryptophan, leucine and isoleucine, for 10 days. The animals were killed two, 5 and 23 hours after feeding. The pancreases and intestinal contents were analyzed for lipase, protease, and amylase activity. Amylase activity was also determined in the plasma. Stomach contents were collected, as a measure of digestion, and carcass composition was determined.

All animals made deficient in a single amino acid lost considerable amounts of weight, but only in the group fed a diet free of isoleucine was the composition of the carcass significantly different from that of the control.

Digestion, as measured by the amount of solids removed from the stomach and small intestine two and 5 hours after feeding, was unimpaired in the deficient animals.

Animals fed diets free of valine, tryptophan, leucine or isoleucine had the least ability to maintain pancreatic enzyme activities during dietary stimulation and were least able to recover pancreatic enzyme activities after a 23-hour fast. A deficiency of lysine had less effect on pancreatic enzyme activities in the secreting and fasted pancreas than did a deficiency of the other 4 amino acids.

Plasma amylase activity did not correlate with the functional capacity of the pancreas, thus indicating that extra-pancreatic sources were probably responsible for this blood enzyme.

1 This investigation was supported in part by grant-in-aid A-3046 from the National Institutes of Health, U. S. Public Health Service, Bethesda, Maryland.

Manuscript received 4 September 1962.




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