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Nutrition of the Pale Western Cutworm, Agrotis orthogonia Morr. (Lepidoptera:Noctuidae)

V. Utilization of Protein Labeled with Carbon-14^1,2,

Canada Agriculture Research Station, Lethbridge, Alberta

A diet containing algal protein-U-C¹⁴ provided a convenient means of studying in vivo protein utilization by larvae of the pale western cutworm Agrotis orthogonia Morr. Utilization of dietary protein in both first- and fifth-instar larvae was found to be quantitatively significant. This technique avoids the difficulty of enzyme extraction artifacts and has particular application to organisms that are too small to permit investigation by classical methods.

² A preliminary report of some of this work was presented at the Fifth International Congress on Nutrition, Washington, D. C., September, 1960.

Manuscript received 31 October 1961.