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The Mechanism of Gossypol Detoxification by Ruminant Animals¹

Department of Biochemistry and Nutrition, Texas Agricultural Experiment Station, College Station, Texas

The indifference of the degree of gossypol binding of rumen liquor to aerobic or anaerobic incubation, high or low temperature, centrifugation or proteolytic enzymes, and the simultaneous disappearance of two moles of lysine -amino groups to each mole of gossypol, demonstrates convincingly that the mechanism of ruminant detoxification of gossypol is by binding to soluble proteins, and that the bond is permanent during protein digestion.

Manuscript received 18 September 1961.

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