Journal of Nutrition EB Program 2010 Early Registration

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dicks, M. W.
Right arrow Articles by Matterson, L. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dicks, M. W.
Right arrow Articles by Matterson, L. D.

Chick Liver-Storage Bioassay of Alpha-Tocopherol: METHODS1,2.3,

Martha W. Dicks4 and L. D. Matterson

Poultry Science Department, Storrs Agricultural Experiment Station, University of Connecticut, Storrs, Connecticut

The results of both a long (two-week supplementation) multiple slope-ratio chick liver-storage bioassay and a short (three-day supplementation, one-day stabilization) bioassay to tocopherol indicated that the different forms of {alpha}-tocopherol were utilized to the same relative degree under the two sets of conditions. The d,{alpha}-tocopheryl acid succinate in relation to the dl,{alpha}-tocopheryl acetate was only 0.61 (short bioassay) to 0.73 and 0.77 (long bioassay) as well utilized by the chick.

The method of Bro-Rasmussen and Hjarde ('57a), when modified as to the saponification procedure, with the same procedure of chromatography through secondary magnesium phosphate, and with a modified method of reading with ferric chloride-dipyridyl, was shown to be a possible substitute for the method of molecular distillation and Florex-chromatography for analysis of liver for {alpha}-tocopherol. Limitations of the method of saponification followed by secondary magnesium phosphate-chromatography were reported.

1 Supported in part by Distillation Products Industries, Rochester, New York, and Yantic Grain and Products Company, Norwich, Connecticut.

2 From a thesis submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree.

3 Presented in part at the Informal Poultry Nutrition Conference in conjunction with the Federation Meetings at Chicago, Illinois, 1960, at the 49th annual meeting of the Poultry Science Association at Davis, California, 1960, and at the annual meeting of the Animal Nutrition Research Council in Washington, D. C., 1960.

4 Present address: Division of Home Economics, University of Wyoming, Laramie, Wyoming.

Manuscript received 17 March 1961.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 1961 by American Society for Nutrition