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Metabolism of Norleucine by the Intact Cow¹

School of Veterinary Medicine and College of Agriculture, University of California, Davis, California

Four lactating cows were injected intravenously with norleucine-3-C¹⁴ or norleucine-2-C¹⁴, and casein was isolated from milk collected three hours later. The specific activities and intramolecular C¹⁴ distribution were determined for several amino acids recovered from casein samples.

The labeling pattern among the amino acids indicated that norleucine metabolism in the cow involves a pathway that includes deamination, decarboxylation, and beta-oxidation of the resulting valerate.

The effect of administering norleucine to cows at increasing levels for 90 days was studied in terms of the utilization of norleucine carbon for amino acid synthesis. The results indicated that the cows adapted to the norleucine in a manner resulting in greater utilization of norleucine carbon for synthesis of glutamic acid, aspartic acid, serine, glycine, and perhaps alanine.

² Present address: Western Regional Research Laboratory, Albany, California.

Manuscript received 20 February 1961.