Journal of Nutrition

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Journal of Nutrition Vol. 74 No. 3 July 1961, pp. 335-341
Copyright © 1961 by American Society for Nutrition
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Essential Fatty Acid Nutrition in Swine

I. Linoleate Requirement Estimated from Triene: Tetraene Ratio of Tissue Lipids1

Eldon G. Hill, E. L. Warmanen, C. L. Silbernick and Ralph T. Holman

The Hormel Institute and Departments of Animal Husbandry and Physiological Chemistry, University of Minnesota, Austin, Minnesota

Sixty-six swine were fed purified diets varying in linoleate content from zero to 12.9% of calories. Polyunsaturated fatty acids were determined in the lipids of the hearts and livers. Characteristic high levels of tissue trienoic acids and low levels of tetraenoic acids were observed in the unsupplemented swine. As dietary linoleate was increased, this relationship was rapidly reversed. The linoleate requirement was deduced from the plot of triene/tetraene ratio versus dietary linoleate. The curves for heart and liver were remarkably similar. From these curves and the weight gains, the dietary linoleate requirement is stated to be near 2% of calories.


1 Supported by grants from the Hormel Foundation, National Vitamin Foundation and National Institutes of Health (grants A-2836 and A-4524).

Manuscript received 8 February 1961.





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