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The Hormel Institute and Departments of Animal Husbandry and Physiological Chemistry, University of Minnesota, Austin, Minnesota
Sixty-six swine were fed purified diets varying in linoleate content from zero to 12.9% of calories. Polyunsaturated fatty acids were determined in the lipids of the hearts and livers. Characteristic high levels of tissue trienoic acids and low levels of tetraenoic acids were observed in the unsupplemented swine. As dietary linoleate was increased, this relationship was rapidly reversed. The linoleate requirement was deduced from the plot of triene/tetraene ratio versus dietary linoleate. The curves for heart and liver were remarkably similar. From these curves and the weight gains, the dietary linoleate requirement is stated to be near 2% of calories.
Manuscript received 8 February 1961.