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Journal of Nutrition Vol. 73 No. 1 January 1961, pp. 38-42
Copyright © 1961 by American Society for Nutrition
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The Amino Acid Composition and Nutritive Value of Proteins

IV. Phenylalanine, Tyrosine, Methionine and Cystine Requirements of the Growing Rat1

P. B. Rama Rao, H. W. Norton and B. Connor Johnson

Division of Animal Nutrition, University of Illinois, Urbana, Illinois

Male weanling rats were fed amino acid diets ad libitum in three-week growth experiments.

The requirements of L-phenylalanine in the presence of excess L-tyrosine (0.7%) and of L-methionine in the presence of excess L-cystine (0.5%) were found to be 0.4 and 0.16% of the diet, respectively, which can be supplied as tyrosine or phenylalanine, and as cystine or methionine.

Rats fed a complete L-amino acid diet containing all the amino acids at their minimal requirement levels in a 10% conventional protein (N x 6.25) ration grew normally.

1 These studies were supported in part under contract no. DA-49-007 MD-544, with the office of the Surgeon General, Department of the Army. The opinions expressed in this publication are those of the authors and not necessarily those of the Department of the Army.

Manuscript received 17 August 1960.




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