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Human Nutrition Research Laboratory, Department of Home Economics, College of Agriculture, University of Nebraska, Lincoln, Nebraska
The availability of isoleucine in corn for human subjects has been determined using experimental diets containing 6 or 10 gm of nitrogen. According to the nitrogen balance data obtained isoleucine in corn seems to be completely available. With an intake of 422 mg of isoleucine daily, nitrogen retention was significantly better in response to isoleucine from corn than to purified isoleucine. The isoleucine requirement appears to be greater than 422 mg per day for women as well as for men. Symptoms of isoleucine deficiency developed within 7 days with a daily intake of 222 mg.
2 Present address: School of Home Economics, University of Wisconsin, Madison.
Manuscript received 23 June 1960.