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Availability to Man of Amino Acids from Foods

IV. Isoleucine from Corn1

Hellen Linkswiler2, Hazel Metz Fox and Peggy Crooke Fry

Human Nutrition Research Laboratory, Department of Home Economics, College of Agriculture, University of Nebraska, Lincoln, Nebraska

The availability of isoleucine in corn for human subjects has been determined using experimental diets containing 6 or 10 gm of nitrogen. According to the nitrogen balance data obtained isoleucine in corn seems to be completely available. With an intake of 422 mg of isoleucine daily, nitrogen retention was significantly better in response to isoleucine from corn than to purified isoleucine. The isoleucine requirement appears to be greater than 422 mg per day for women as well as for men. Symptoms of isoleucine deficiency developed within 7 days with a daily intake of 222 mg.


1 This study was part of a North Central Regional Project (NC-49, Factors affecting requirements of adult human subjects for protein and amino acids), a cooperative study involving agricultural experiment stations in the North Central Region and supported in part by regional funds. Published with the approval of the Director as Paper no. 1045, Journal Series, Nebraska Agricultural Experiment Station.

2 Present address: School of Home Economics, University of Wisconsin, Madison.

Manuscript received 23 June 1960.





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