Journal of Nutrition

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Journal of Nutrition Vol. 71 No. 2 June 1960, pp. 159-167
Copyright © 1960 by American Society for Nutrition
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Biosynthesis of Sterols and Fatty Acids as Affected by Nicotinic Acid and Related Compounds1

R. W. F. Hardy2, J. L. Gaylor3 and C. A. Baumann

Department of Biochemistry, University of Wisconsin, Madison

1. The incorporation of acetate-1-C14 into sterols and fatty acids was examined with liver slices from rats and chicks fed nicotinic acid (NAc), nicotinamide (NAm), isonicotinic acid (INA) or benzoic acid; these compounds were also added to liver slices in vitro.
2. Liver from rats or chicks fed NAc or related compounds showed an increased incorporation of acetate into sterols and a decreased incorporation into fatty acids. This altered incorporation varied with the length of time during which NAc or related compounds had been fed.
3. Incubations of rat liver slices in a buffer 0.01 to 0.10 M in NAc, NAm, or INA resulted in substantial decreases in fatty acid synthesis, the most marked depressions occurring with INA; this was accompanied at certain concentrations by increases in sterol synthesis, the more marked elevations occurring with NAc. Significant depressions in fatty acid synthesis with 0.05 M NAc, NAm or INA occurred before changes in sterol synthesis were detectable.
4. These results, plus observations by others, suggest that a primary change in fatty acid synthesis may determine an altered partition of acetate between fatty acids and sterols.

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by grant C-2177 of the National Institutes of Health.

2 duPont Fellow 1958–1959. Present address: Central Research Department, Experimental Station, E. I. duPont de Nemours and Co., Wilmington, Delaware.

3 P.H.S. Research Fellow of the National Cancer Institute.

Manuscript received 22 January 1960.




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