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Journal of Nutrition Vol. 71 No. 2 June 1960, pp. 115-121
Copyright © 1960 by American Society for Nutrition
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Chick Tissue-Storage Bioassay of Alpha-Tocopherol: Chemical Analytical Techniques, and Relative Biopotencies of Natural and Synthetic Alpha-Tocopherol1

W. J. Pudelkiewicz, L. D. Matterson, L. M. Potter, Lorna Webster and E. P. Singsen

Poultry Science Department, Storrs Agricultural Experiment Station, University of Connecticut, Storrs

A chick bioassay method is described for comparing the potencies of d and dl, {alpha}-tocopherol with their respective acetate esters. It was found that d,{alpha}-tocopheryl acetate was 1.34 times more potent than dl,{alpha}-tocopheryl acetate as measured by liver tocopherol content, and d or dl,{alpha}-tocopherol were utilized equally as well as their respective acetate esters.

1 Supported in part by grants from the Yantic Grain and Products Company, Norwich, Connecticut, and Hoffmann-LaRoche, Inc., Nutley, New Jersey.

Manuscript received 23 December 1960.




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