QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hardy, R. W. F. Articles by Baumann, C. A. Search for Related Content PubMed PubMed Citation Articles by Hardy, R. W. F. Articles by Baumann, C. A.

Effects of Amino Acids on the Excretion of Various Proteins by the Rat¹

Department of Biochemistry, University of Wisconsin, Madison

1. Rats were given large doses of single amino acids by stomach tube, and measurements were made of urinary protein, urea and -amino nitrogen. Individual urinary proteins were determined after separation by paper or starch-gel electrophoresis, or column chromatography.

2. Most of the amino acids increased protein excretion roughly in proportion to the increased urea production. DL-Serine, D-serine and L-cysteine·HCl produced excessive elevations in protein excretion, whereas glycine and alanine depressed it.

3. Essentially similar distributions of the urinary proteins resulted from separations by paper or starch-gel electrophoresis or on a cellulose column. With respect to blood proteins, urinary proteins are low in albumin and -globulins and high in - and ß-globulins.

4. The administration of most of the amino acids failed to alter the distribution of the urinary proteins. However, after the administration of DL-serine the pattern approached that of plasma (albumin and -globulins increased). The administration of glycine to male rats yielded a pattern roughly similar to that in normal female urine (albumin and -globulins depressed).

² DuPont Fellow, 1958–59.

³ Appointment supported by the International Cooperation Administration administered by the National Academy of Sciences of America.

Manuscript received 25 November 1959.