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Biological Availability of Amino Acids in Fish Meals and Other Protein Sources¹

Department of Poultry Husbandry, University of California and Bureau of Commercial Fisheries, Davis

A new chick assay method is proposed for the estimation of essential amino acids available to the animal from various protein sources. The material to be tested is fed as a supplement to a diet replete with crystalline forms of all essential amino acids except the one being studied; thus the source supplies this one as well as non-essential amino acid needs. The growth response to this diet, compared with that for the same diet plus the missing amino acid, allows estimation of the amino acids furnished by the protein source. The method has been used successfully for lysine, methionine and cystine, tryptophan, phenylalanine, histidine, and threonine. It has been less successful for arginine, and unsatisfactory for glycine, isoleucine, leucine, and valine. Results of tests with 19 protein sources are given for a number of amino acids, with special emphasis on fish meals of variable and known histories.

² Present address: Department of Veterinary Science, University of California, Davis.

Manuscript received 24 February 1959.