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U. S. Army Medical Research and Nutrition Laboratory Fitzsimons Army Hospital, Denver
An army C ration that had been stored (after assembly) for 20 months at 34°F. contained an average of 2.76 mg of vitamin B6 per ration by microbiological assay. Other rations from the same assembly that had been stored for the same length of time at 100°F. contained an average of 1.93 mg of vitamin B6.
These two C rations, together with a ration of similar macronutrient composition prepared from fresh food, were fed for 24 days each to 9 young male test subjects. At the end of the period of consumption of the C rations, the average net excretion of xanthurenic acid, after ingestion of 10 gm of DL-tryptophan, was 11.6 mg in 24 hours in the case of the ration stored at 34°F., and 31.9 mg for the ration stored at 100°F. as compared to 11.3 mg for the control ration.
Under the conditions of the experiment the minimum daily requirements of the test subjects for vitamin B6 was between 1.93 and 2.76 mg.