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Studies of Vitamin B₁₂ in Turnip Greens

U. S. Plant, Soil and Nutrition Laboratory, Agricultural Research Service, U.S.D.A., Ithaca, N. Y.; and the Department of Biochemistry and Nutrition, Cornell University, Ithaca, N. Y.

The presence in turnip greens of a substance with vitamin B₁₂ activity for chicks and for Ochromonas malhamensis was demonstrated.

The possible identity of this active substance with vitamin B₁₂ was investigated using paper electrophoresis and chromatography. By these criteria, there was no evidence that the substance in the turnip greens differed from cyanocobalamin.

The fact that the vitamin B₁₂-active material was found in turnip greens grown at one location but not in those from another raises the question of its origin. Possible sources of this vitamin B₁₂ were discussed.