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The Activity of Certain Water-Soluble Vitamins After Exposure to Gamma Radiations in Dry Mixtures and in Solutions

Department of Biochemistry and Nutrition, Texas Agricultural Experiment Station, College Station

The effect of ionizing radiations on choline, folic acid, thiamine, riboflavin, pyridoxine and pantothenic acid was investigated using baby chicks as experimental animals. The vitamins were irradiated under various conditions with a dose of 2.79 x 10⁶ rad of gamma rays.

There was no evidence that any of the vitamins were destroyed when the synthetic diet containing the vitamins or when a mixture of the vitamins with vitamin-free casein was irradiated. Choline was not destroyed when an aqueous solution containing 300 mg of choline chloride per ml was irradiated. Twenty-five to 50% of the folic acid in a solution containing 50.0 µg/ml was lost by the irradiation process. There was no evidence of destruction of thiamine, riboflavin, pyridoxine, pantothenic acid and folic acid when the vitamins were mixed with casein and irradiated dry, but there was some destruction of each vitamin when irradiation was carried out in an aqueous solution. The loss of thiamine in the solution was largest and the loss of pyridoxine was slightly higher than that of riboflavin and pantothenic acid.