Journal of Nutrition

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Journal of Nutrition Vol. 64 No. 4 April 1958, pp. 625-634
Copyright © 1958 by American Society for Nutrition
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Effect of Age, Sex and Feeding Regimen on Fat Digestibility in Individual Rats as Determined by a Rapid Extraction Procedure

Robert L. Squibb1, Alvaro Aguirre2, J. Edgar Braham3, Nevin S. Scrimshaw3 and Edwin Bridgforth4

Instituto de Nutrición de Centro América y Panamá (INCAP), Guatemala, C. A.

A rapid procedure for the extraction of the fecal fat of rats used in digestion trials is presented. Data obtained by this method demonstrate a marked variation among individual rats of both sexes in the digestion of fat. This variation appears to be greater in adult males; however, restricting the fat intake of the adult male to that of the female lessened the variation. When fed ad libitum, mature females digested more fat than males. This sex difference was not apparent when the fat intake of the males was restricted to that of the females or when young growing rats of either sex were kept on ad libitum or restricted feeding regimens. Since variations in utilization were found to occur with the type of fat, sex and age of the rats and feeding regimen, the importance of standardizing procedures for determining fat digestion coefficients with rats is indicated.

1 Consultant in Animal Nutrition, Instituto de Nutrición de Centro América y Panamá (INCAP), Guatemala, C. A. Present address, Department of Poultry Science, Rutgers University.

2 INCAP Fellow, assigned to the Instituto Agropecuario Nacional (IAN) and Servicio Cooperativo Interamericano de Agricultura (SCIDA), Guatemala, C. A.

3 Director, Instituto de Nutrición de Centro Amércia y Panamá. A cooperative Institute for the study of human nutrition, supported by the Governments of Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua and Panama and administered by the Pan American Sanitary Bureau, Regional Office of the World Health Organization.

4 Acting Chief, Division of Statistics, INCAP, and Assistant Professor of Biostatistics, Vanderbilt University, School of Medicine. INCAP Scientific Publication I-46.

Manuscript received 8 July 1957.




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