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Comparative Measurements of Ascorbic Acid and Total Ascorbic Acid of Blood Plasma^1,2,

College of Home Economics and Agricultural Experiment Station, The Pennsylvania State University, University Park

A comparison was made of micro determinations of ascorbic and total ascorbic acid contents of 61 non-hemolyzed samples of plasma. The measurement of total ascorbic acid averaged 0.18 mg per 100 ml higher than that of ascorbic acid in the same plasma. The difference between the two analyzed forms in plasma was statistically highly significant. While the higher values for total than for ascorbic acid are not considered to be conclusive evidence for the presence of dehydroascorbic acid in circulating blood, they do indicate that a correction should be applied if direct comparisons are desired between values for plasma ascorbic acid and levels of the vitamin reported as total ascorbic acid.

² Research Contribution no. 153, College of Home Economics.

³ Presented from a dissertation submitted to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirement for the Doctor of Philosophy degree.

Manuscript received 21 September 1957.