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Journal of Nutrition Vol. 64 No. 1 January 1958, pp. 99-111
Copyright © 1958 by American Society for Nutrition
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Nitrogen Balances of Women Maintained on Various Levels of Methionine and Cystine1

May S. Reynolds, Dorothy L. Steel2, Evelyn M. Jones3, C. A. Baumann, Eleanor Halter, Miriam McTeer, Saranya Reddy, Frances Schmidt and Gertrude Skerski

Department of Foods and Nutrition, School of Home Economics and Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

With a cystine intake of 500 mg, 150 to 180 mg of methionine resulted in nitrogen storage in 4 of 7 women, while the balances of the other three were within the "zone of equilibrium." An intake of 290 mg appeared to be adequate for all 13 subjects studied at those levels. A total of 300 mg of the two sulfur-containing amino acids appeared to be marginal while 550 mg resulted in some nitrogen storage in all subjects. From these data it appears that a total of 550 mg of the sulfur-containing amino acids is adequate for the establishment of nitrogen balance in women, under these conditions.

1 Contribution no. 14, Subproject 1, "Protein and Amino Acid Requirements in Human Metabolism," of the North Central Region Cooperative Project NC-5, "Nutritional Status and Dietary Needs of Population Groups." Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by a U. S. Department of Agriculture contract, sponsored by the Human Nutrition Research Division, Agricultural Research Service. Presented in part before the 19th meeting of the American Institute of Nutrition, San Francisco, April 1955 (Fed. Proc., 14: 438, 1955). Parts of the data in this paper are taken from theses submitted by Dorothy L. Steel and Evelyn M. Jones in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

2 Present address: Division of Home Economics, Oklahoma A and M College, Stillwater, Oklahoma.

3 Present address: Department of Foods and Nutrition, College of Home Economics, Michigan State University, East Lansing.

Manuscript received 22 July 1957.


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