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Necrogenic Potency of Yeasts Grown on Various Media¹

Departments of Biochemistry and Poultry Husbandry, College of Agriculture, University of Wisconsin, Madison

1. Death due to hepatic necrosis occurred rapidly and in 100% incidence in vitamin E-depleted rats fed Torulopsis utilis grown on spent sulfite liquor as the sole source of protein. Comparable diets containing brewers' by-products yeast did not produce necrosis. Survival was increased by increasing the level of the torula-SSL yeast in the diet.

2. Torulopsis utilis and Saccharomyces cerevisiae grown on a synthetic medium were both found to be necrogenic, while these same yeasts grown on commercial beer wort were both found to be non-necrogenic.

3. Liver necrosis was prevented by the addition of methylene blue or diphenyl-p-phenylenediamine (DPPD) to the diet; butylated hydroxytoluene was ineffective.

Manuscript received 19 June 1957.