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Nutrition of Salmonoid Fishes

III. Water-Soluble Vitamin Requirements of Chinook Salmon¹

U. S. Fish and Wildlife Service, Salmon Nutrition Laboratory, Cook, Washington

A vitamin-test diet consisting of a vitamin-free basal ration supplemented with crystalline vitamins was formulated which would maintain chinook salmon fingerlings for a period sufficient for the development of water-soluble vitamin deficiency syndromes.

Deleting the water-soluble vitamins one at a time from the complete test diet caused typical specific deficiency syndromes in chinook salmon which were comparable to those observed in other experimental animals.

Requirements for thiamine, riboflavin, pyridoxine, pantothenic acid, niacin, inositol, biotin, folic acid and choline were demonstrated.

Inconclusive results were obtained for vitamin B₁₂-deficient fish.

Under the experimental conditions used, no deficiency syndromes were observed in ascorbic acid- or para-aminobenzoic acid-deficient lots of salmon.

Manuscript received 28 December 1956.