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Further Evidence on the Requirement of the Chick for Unidentified Minerals¹

Agricultural Experiment Station and School of Nutrition, Cornell University, Ithaca, New York

In further work on unidentified chick growth factors results were obtained which indicated that the basal diets supplied the chicks were adequate in amino acids, known vitamins and minerals, previously reported to be needed by animals. No evidence of an imbalance was obtained in the studies on required minerals. As a consequence it has been found, in confirmation of previous reports by this laboratory, that when a mixture of unidentified growth factor supplements is fed to chicks the growth response which is observed is due to the presence in the materials of both unidentified organic and inorganic constituents. The results of the investigation showed, therefore, that a mineral or minerals not hitherto considered to be essential in the nutrition of the chick is present in the mineral portion or ash of certain crude feedstuffs. The unidentified mineral(s) was found to be involved in bone formation. Evidence was also obtained which indicated that the unknown mineral nutrient(s) is present in the boiling water-insoluble fraction of the ash of the mixture of unidentified factor supplements, and that it is cationic in acid solution. No consistent effect of the ash on the intestinal microflora of the chicks or the pH of the intestinal contents was observed.

Manuscript received 17 May 1956.