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A Study of the Effect of Chlortetracycline (Aureomycin) Upon Calcium Retention by the Growing Male Albino Rat¹

Department of Animal Nutrition, The Pennsylvania State University, University Park

Ten replicates of 4 weanling, male, albino rats each were fed daily for a 35-day period equal quantities of the following experimental diets: rat 1 received the basal diet (containing approximately 18% casein, 0.29% Ca and 0.44% P), rat 2 received the basal diet + aureomycin (100 mg/kg), rat 3 received the basal diet + additional Ca (0.55% Ca), and rat 4 received the basal diet + additional Ca and aureomycin.

Calcium supplementation of the basal diet resulted in a highly significant increase (22.5%) in carcass Ca gain, a significant decrease (2.7%) in body weight gain, a highly significant decrease (7.3% and 35.6%, respectively) in carcass water gain and in the percentage of Ca retention.

Aureomycin supplementation of the basal diet did not increase body weight gain, carcass water gain, body length, carcass Ca gain, or the percentage of Ca retention. Aureomycin, did, however, increase (10.7%) the carcass ether extract gain to an extent that closely approached statistical significance at the 5% level, and significantly decreased (3.5%) carcass dry matter gain.

Manuscript received 20 March 1956.