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Department of Home Economics, College of Agriculture, University of Illinos Urbana
Storage of frozen serum samples for periods of 4 and 22 weeks and thawing of these samples as often as three times did not alter the concentrations of the free and total serum cholesterol when compared with the values in fresh samples.
A mean total serum cholesterol concentration of 249 mg% was observed for 113 women from 26 to 92 years of age. The average cholesterol content of the serum increased gradually from 207 mg % to a maximum of 293 mg % at a mean age of 27.3 and 54.2 years, respectively. This maximum value was maintained during the 6th and 7th decades of life. Thereafter, the concentration of cholesterol declined to 239 mg % at 77.6 years of age. The ratio of the free to total cholesterol was constant for all age groups.
Significant positive correlations for age and relative body weight but a negligible correlation for the diastolic blood pressure with the total serum cholesterol was found. The percentage of dietary fat and protein calories as well as the total energy intake did not show a significant relationship with the variation in total blood cholesterol.
2 Present address is 2110 N. 89th Streeth, Seattle 3, Washington.
3 The authors wish to express their appreciation to Winifred Newton for here technical assistance.
Manuscript received 11 November 1955.