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The Effects of Dietary Manganese and Thiamine Levels on Growth Rate and Manganese Concentration in Tissues of Rats^1,2,

Department of Biochemistry, Purdue University Agricultural Experiment Station, West Lafayette, Indiana

The time required to deplete rats of thiamine was not significantly altered by raising the manganese content of the diet.

The addition of either thiamine or manganese to diets deficient in both of these nutrients improved the growth rate of rats but there was no significant interaction between the effects of these two nutrients on the growth rate.

The amount of manganese present in heart and liver tissue was found to vary directly with the manganese level in the diet and was not significantly affected by the level of thiamine in the diet.

² This investigation was supported in part by a grant from Lilly Endowment, Inc.

³ Present address: McCollum-Pratt Institute, Johns Hopkins University, Baltimore 18, Md.

Manuscript received 19 March 1955.