Journal of Nutrition

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Journal of Nutrition Vol. 56 No. 4 August 1955, pp. 545-554
Copyright © 1955 by American Society for Nutrition
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Nitrogen Utilization During Caloric Restriction

II. The Effect of Variation in Nitrogen Intake1

One Figure

Doris Howes Calloway and Harry Spector

Quartermaster Food and Container Institute for the Armed Forces, Chicago, Illinois

Diets supplying graded amounts of egg albumin nitrogen, zero to 604 mg per day, were fed to normal adult rats limited to 50% of caloric requirement for 4 days. Nitrogen utilization was measured by nitrogen balance and by alteration in liver nitrogen content.

The degree of negative nitrogen balance was inversely proportional to nitrogen intake but positive balance was not attained. Nitrogen utilization diminished as the level of dietary nitrogen increased from 52 to 61% at 70 to 80 mg daily to 17% at 604 mg, indicating increased use of dietary protein for energy. Supplying half the caloric requirement reduced the nitrogen deficit 32 to 39% as compared with fasting and the addition of dietary nitrogen produced up to 87% sparing beyond that of the calories alone.

There was no significant correlation between liver nitrogen loss and nitrogen balance.


1 This paper reports research undertaken at the Quartermaster Food and Container Institute for the Armed Forces, and has been assigned no. 529 in the series of papers approved for publication. The views or conclusions contained in this report are those of the authors. They are not to be construed as necessarily reflecting the views or indorsement of the Department of Defense.

Manuscript received 14 February 1955.





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